AmyX

• Description: pullulanase (debranching enzyme)
  
  

Gene name	amyX
Synonyms
Essential	no
Product	pullulanase
Function	starch degradation
MW, pI	80 kDa, 5.799
Gene length, protein length	2154 bp, 718 aa
Immediate neighbours	ytmP, ytlR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): GlgB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2E8Z (complex with alpha cyclodextrin), 2E9B (complex with maltose)

• Swiss prot entry:

• KEGG entry: BSU29930

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jae-Hoon Shim, Jong-Tae Park, Jung-Sun Hong, Ki Woo Kim, Myo-Jeong Kim, Jung-Hyuk Auh, Young-Wan Kim, Cheon-Seok Park, Winfried Boos, Jung-Wan Kim, Kwan-Hwa Park
Role of maltogenic amylase and pullulanase in maltodextrin and glycogen metabolism of Bacillus subtilis 168.
J Bacteriol: 2009, 191(15);4835-44
[PubMed:19465663] [WorldCat.org] [DOI] (I p)

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed