PbuG

• Description: hypoxanthin/ guanine permease
  
  

Gene name	pbuG
Synonyms	yebB
Essential	no
Product	hypoxanthin/ guanine permease
Function	hypoxanthine and guanine uptake
MW, pI	46 kDa, 8.808
Gene length, protein length	1320 bp, 440 aa
Immediate neighbours	guaA, yebC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): PbuO

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane PubMed

Database entries

• Structure: 2EEW (the pbuG riboswitch, U47c mutant, bound to hypoxanthine)

• Swiss prot entry:

• KEGG entry: BSU06370

• E.C. number:

Expression and regulation

• Operon: pbuG

• Sigma factor:

• Regulation:
  • repressed by hypoxanthine and guanine, PurR-independent, (G-box)PubMed
  • repressed in the presence of adenine or adenosine(PurR) PubMed

• Regulatory mechanism:
  • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • G-box: transcription termination/ antitermination (riboswitch) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
2. Johansen et al. (2003) Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL). J. Bacteriol. 185: 5200-5209. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed