GltC

• Synonyms:

• Description: Transcriptional activator of the gltAB operon. Activates expression of the operon in the absence of arginine.

The gene

Basic information

• Coordinates:

• Gene length:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription activation of the gltAB operon

• Protein family: LysR-type transcription regulator

• Paralogous protein(s): none, but there are 19 members of the LysR family in B. subtilis

Database entries

• Structure

• Swiss prot entry

• KEGG entry

Extended information on the protein

• Kinetic information

• Domains DNA-binding helix-turn-helix motif: AA 18 ... 37

• Modification

• Effectors of protein activity:2-oxoglutarate

• Interactions:
  • GltC-RocG, This interaction takes place in the presence of glutamate. It prevents the transcription activation of the gltAB operon. Note that RocG expression is strongly regulated. <ref>Commichau et al. (2007)</ref>

• Localization

Expression and Regulation

• Operon: [3]

• Sigma factor: SigA [4]

• Regulation: autoregulation by GltC [5]

• Regulatory mechanism: autorepression

Biological Materials

• Mutant:, Expression vector

• Expression vector:

• lacZ fusion:

• GFP fusion:

• Antibody: available in Stülke lab

Additional remarks

• Labs working on this gene/protein:

Linc Sonenshein, Tufts University, Boston, MA, USA [6]

Jörg Stülke, University of Göttingen, Germany [7]