PhoP

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  • Description: two-component response regulator, regulation of phosphate metabolism

Gene name phoP
Synonyms
Essential no
Product two-component response regulator
Function regulation of phosphate metabolism
(phoA, phoB, phoD, resABCDE, tagAB, tagDEF, tuaA-H)
MW, pI 27 kDa, 5.068
Gene length, protein length 720 bp, 240 aa
Immediate neighbours phoR, mdh
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PhoP context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Genes controlled by PhoP

  • Repression by PhoP:

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation by PhoR under conditions of phosphate limitation (stimulates DNA-binding activity)
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure: 1MVO (receiver domain)
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: repressed by glucose (4.3-fold) (CcpA) PubMed, induced by phosphate limitation (PhoP), carbon catabolite repression (CcpA)
  • Regulatory mechanism: CcpA: transcription repression, PhoP: transcription activation, CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed