pabA

168

para-aminobenzoate synthase (subunit B)/ anthranilate synthase (subunit II), glutaminase subunit

Locus: BSU_00750

Molecular weight: 21.54 kDa

Isoelectric point: 4.78

Protein length: 194 aa

Gene length: 585 bp

Function: biosynthesis of folate and tryptophan

Product: para-aminobenzoate synthase (subunit B)/ anthranilate synthase (subunit II) glutamine amidotransferase (subunit B) and anthranilate synthase (subunit II)

Essential: no

E.C.: 2.6.1.85

Synonyms: pabA, trpG, trpX

Genomic Context

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Categories containing this gene/protein

Metabolism, Amino acid/ nitrogen metabolism, Biosynthesis/ acquisition of amino acids, Biosynthesis/ acquisition of aromatic amino acids

Metabolism, Additional metabolic pathways, Biosynthesis of cofactors, Biosynthesis of folate

List of homologs in different organisms, belongs to COG0512 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon

Gene

Coordinates: 84,290  84,874

The protein

Catalyzed reaction/ biological activity

chorismate + L-glutamine --> anthranilate + pyruvate + L-glutamate (according to UniProt)

Domains

Glutamine amidotransferase type-1 domain (aa 1-194) (according to UniProt)

Structure

6QUR (PDB) (synthetic construct, 72.9% identity) PubMed
1I1Q (PDB) (from Salmonella typhimurium, 42% identity) PubMed
P28819 (AlphaFold)

Expression and Regulation

Operons

Genes: pabB-pabA-pabC-sul-folB-folK-yazB-dusB1-lysS

Description: PubMed

Regulation

translation repressed by tryptophan (MtrB) PubMed

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Other regulations

MtrB: translation inhibition, PubMed

Additional information

the pabA-pabC part of the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

BKE00750 (pabA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAAATCATTTCTCCGC,  downstream forward: _UP4_TATCGCAAGGAAGTTATTGC
BKK00750 (pabA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAAATCATTTCTCCGC,  downstream forward: _UP4_TATCGCAAGGAAGTTATTGC

References

Reviews

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Original Publications

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