PdhD

• Description: dihydrolipoamide dehydrogenase E3 subunit of both pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes
  
  

Gene name	pdhD
Synonyms	citL
Essential	no
Product	dihydrolipoamide dehydrogenase E3 subunit of both pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes
Function	links glycolysis and TCA cycle, enzyme in TCA cycle
MW, pI	49 kDa, 4.76
Gene length, protein length	1410 bp, 470 aa
Immediate neighbours	pdhC, slp
Gene sequence (+200bp)	Protein sequence
Genetic context

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

defects in sporulation and unable to grow on glucose as single carbon source PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: PdhA-PdhB-PdhC-PdhD, OdhA-OdhB-PdhD

• Localization:

Database entries

• Structure: 1EBD (complex with binding domain of dihydrolipoamide acetylase, Geobacillus stearothermophilus)

• Swiss prot entry:

• KEGG entry: [3]

• E.C. number: 1.8.1.4

Expression and regulation

• Operon: pdhA-pdhB-pdhC-pdhD

• Sigma factor: SigA

• Regulation: weak induction by glucose PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Gao et al. (2002) The E1beta and E2 subunits of the Bacillus subtilis pyruvate dehydrogenase complex are involved in regulation of sporulation.J. Bacteriol. 184: 2780-2788. PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed