PtkA

• Description: write here
  
  

Gene name	ptkA
Synonyms	ywqD
Essential	no
Product
Function	unknown
MW, pI	25 kDa, 9.628
Gene length, protein length	711 bp, 237 aa
Immediate neighbours	ptpZ, tkmA
Gene sequence (+200bp)	Protein sequence
Genetic context

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: BY-kinase

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: autophosphorylation at residues Y225, Y227 and Y228 (primary site)

• Cofactor(s): ATP

• Effectors of protein activity: TkmA - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry:

• E.C. number:

Expression and regulation

• Operon: tkmA-ptkA-ptpZ-ywqF PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: KO strain created with pMUTIN-2, available from Ivan Mijakovic

• Expression vector: pQE-30, N-terminally 6xHis-tagged, available from Ivan Mijakovic

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Ivan Mijakovic, Thiverval-Grignon, France

References

1. Mijakovic I, Poncet S, Boël G, Mazé A, Gillet S, Decottignies P, Grangeasse C, Jamet E, Doublet P, Le Maréchal P, Deutscher J (2003) Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases. EMBO J 22:4709-4718. PubMed
2. Mijakovic I, Petranovic D, Deutscher J (2004) How tyrosine phosphorylation affects the UDP-glucose dehydrogenase activity of Bacillus subtilis YwqF. J Mol Microbiol Biotechnol 8:19-25. PubMed
3. Mijakovic I, Petranovic D, Macek B, Cepo T, Mann M, Davies J, Jensen PR, Vujaklija D (2006) Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. Nucl Acids Res 34:1588-1596. PubMed
4. Petranovic D, Michelsen O, Zahradka K, Silva C, Petranovic M, Jensen PR, Mijakovic I (2007) Bacillus subtilis strain deficient for the protein-tyrosine kinase PtkA exhibits impaired DNA replication. Mol Microbiol 63:1797-1805. PubMed