HolA

• Description: DNA polymerase III, delta subunit, part of the clamp-loader complex and the replisome
  
  

Gene name	holA
Synonyms	yqeN
Essential	yes PubMed
Product	DNA polymerase III, delta subunit
Function	DNA replication
Gene expression levels in SubtiExpress: holA
Interactions involving this protein in SubtInteract: HolA
MW, pI	40 kDa, 6.231
Gene length, protein length	1041 bp, 347 aa
Immediate neighbours	rpsT, yqzM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU25560

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU25560

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for bacteriophage SPP1 replication PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • domain I: aa 1 - 143, RecA-like fold PubMed
  • domain II: aa 144 - 214, four alpha helices PubMed
  • domain III: aa 215 - 339, seven alpha helices PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:#
  • DnaX(3)-HolA-HolB, the clamp-loader complex PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • DnaE-HolA PubMed

• Localization: Nucleoid (Mid-cell) PubMed

Database entries

• BsubCyc: BSU25560

• Structure: 3ZH9 PubMed

• UniProt: P54459

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: holA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Olivier Rannou, Emmanuelle Le Chatelier, Marilynn A Larson, Hamid Nouri, Bérengère Dalmais, Charles Laughton, Laurent Jannière, Panos Soultanas
Functional interplay of DnaE polymerase, DnaG primase and DnaC helicase within a ternary complex, and primase to polymerase hand-off during lagging strand DNA replication in Bacillus subtilis.
Nucleic Acids Res: 2013, 41(10);5303-20
[PubMed:23563155] [WorldCat.org] [DOI] (I p)

José P Afonso, Kiran Chintakayala, Chatrudee Suwannachart, Svetlana Sedelnikova, Kevin Giles, John B Hoyes, Panos Soultanas, John B Rafferty, Neil J Oldham
Insights into the structure and assembly of the Bacillus subtilis clamp-loader complex and its interaction with the replicative helicase.
Nucleic Acids Res: 2013, 41(9);5115-26
[PubMed:23525462] [WorldCat.org] [DOI] (I p)

Elena M Seco, John C Zinder, Carol M Manhart, Ambra Lo Piano, Charles S McHenry, Silvia Ayora
Bacteriophage SPP1 DNA replication strategies promote viral and disable host replication in vitro.
Nucleic Acids Res: 2013, 41(3);1711-21
[PubMed:23268446] [WorldCat.org] [DOI] (I p)

Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408] [WorldCat.org] [DOI] (I p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)