PgpH

• Description: c-di-AMP specific phosphodiesterase
  
  

Gene name	pgpH
Synonyms	yqfF
Essential	no
Product	c-di-AMP specific phosphodiesterase
Function	control of c-di-AMP homeostasis
Gene expression levels in SubtiExpress: pgpH
MW, pI	78 kDa, 7.697
Gene length, protein length	2133 bp, 711 aa
Immediate neighbours	yqfG, phoH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides, membrane proteins

The gene

Basic information

• Locus tag: BSU25330

Database entries

• BsubCyc: BSU25330

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • hydrolysis of c-di-AMP to 5'-pApA PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors: Mn(2+) PubMed

• Effectors of protein activity:
  • ppGpp acts as allosteric inhibitor of phosphodiesterase activity (in Listeria monocytogenes) PubMed

• Interactions:
  • PgpH is a member of a suspected group of hubs proteins that were suggested to be involved in a large number of interactions PubMed

• Localization:
  • cell membrane PubMed (according to UniProt)

Database entries

• BsubCyc: BSU25330

• Structure:
  • 4S1B (the HD domain of the protein from Listeria monocytogenes in complex with c-di-AMP, 58% identity, 85% similarity) PubMed

• UniProt: P46344

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: pgpH PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • SM-GN1 (yqfF-spc), available in Anne Galinier's and Boris Görke's labs
  • BKE25330 (yqfF::erm without terminator, available in the BGSC and in Jörg Stülke's lab)
  • GP2033 (yqfF::tet, available in Jörg Stülke's lab)
  • GP2034 (yqfF::erm without terminator, available in Jörg Stülke's lab)
  • GP2049 (yqfF::cat without terminator, available in Jörg Stülke's lab)

• Expression vector:

• lacZ fusion: pGP190 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Fabian M Commichau, Achim Dickmanns, Jan Gundlach, Ralf Ficner, Jörg Stülke
A jack of all trades: the multiple roles of the unique essential second messenger cyclic di-AMP.
Mol Microbiol: 2015, 97(2);189-204
[PubMed:25869574] [WorldCat.org] [DOI] (I p)

John D Helmann
Chemical proteomics reveals a second family of cyclic-di-AMP hydrolases.
Proc Natl Acad Sci U S A: 2015, 112(7);1921-2
[PubMed:25637595] [WorldCat.org] [DOI] (I p)

Original publications

Jan Gundlach, Felix M P Mehne, Christina Herzberg, Jan Kampf, Oliver Valerius, Volkhard Kaever, Jörg Stülke
An Essential Poison: Synthesis and Degradation of Cyclic Di-AMP in Bacillus subtilis.
J Bacteriol: 2015, 197(20);3265-74
[PubMed:26240071] [WorldCat.org] [DOI] (I p)

TuAnh Ngoc Huynh, Shukun Luo, Daniel Pensinger, John-Demian Sauer, Liang Tong, Joshua J Woodward
An HD-domain phosphodiesterase mediates cooperative hydrolysis of c-di-AMP to affect bacterial growth and virulence.
Proc Natl Acad Sci U S A: 2015, 112(7);E747-56
[PubMed:25583510] [WorldCat.org] [DOI] (I p)

Elodie Marchadier, Rut Carballido-López, Sophie Brinster, Céline Fabret, Peggy Mervelet, Philippe Bessières, Marie-Françoise Noirot-Gros, Vincent Fromion, Philippe Noirot
An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach.
Proteomics: 2011, 11(15);2981-91
[PubMed:21630458] [WorldCat.org] [DOI] (I p)