SR4

• Description: antitoxin RNA, inhibits bsrG translation and causes destabilization of the bsrG mRNA
  
  

Gene name	SR4
Synonyms
Essential	no
Product	antitoxin RNA
Function	inhibition of BsrG toxin expression
Gene length	180 bp
Immediate neighbours	bsrG, yokL
Get the DNA sequence
Genetic context File:BsrG context.gif This image was kindly provided by SubtiList

Categories containing this gene/protein

toxins, antitoxins and immunity against toxins, SPß prophage, ncRNA

The gene

Basic information

• Locus tag:

Phenotypes of a mutant

• cell lysis on agar plates PubMed

Database entries

• BsubCyc: ?

• DBTBS entry: no entry

• SubtiList entry: no entry

• Structure:

The RNA

binds the bsrG mRNA and triggers its degradation PubMed, moreover SR4 inhibits bsrG translation PubMed

Expression and regulation

• Operon: SR4 PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • half-life of the mRNA: 3 min PubMed
  • maturation of the RNA requires PnpA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

Labs working on this gene/RNA

Sabine Brantl, Jena, Germany

References

Natalie Jahn, Sabine Brantl, Henrik Strahl
Against the mainstream: the membrane-associated type I toxin BsrG from Bacillus subtilis interferes with cell envelope biosynthesis without increasing membrane permeability.
Mol Microbiol: 2015, 98(4);651-66
[PubMed:26234942] [WorldCat.org] [DOI] (I p)

Natalie Jahn, Sabine Brantl
One antitoxin--two functions: SR4 controls toxin mRNA decay and translation.
Nucleic Acids Res: 2013, 41(21);9870-80
[PubMed:23969414] [WorldCat.org] [DOI] (I p)

Natalie Jahn, Heike Preis, Christoph Wiedemann, Sabine Brantl
BsrG/SR4 from Bacillus subtilis--the first temperature-dependent type I toxin-antitoxin system.
Mol Microbiol: 2012, 83(3);579-98
[PubMed:22229825] [WorldCat.org] [DOI] (I p)