HemAT

• Description: soluble chemotaxis receptor, heme-containing O(2) sensor protein
  
  

Gene name	hemAT
Synonyms	yhfV
Essential	no
Product	haem-based aerotactic transducer
Function	movement towards oxygen
Gene expression levels in SubtiExpress: hemAT
Interactions involving this protein in SubtInteract: HemAT
MW, pI	48 kDa, 5.441
Gene length, protein length	1296 bp, 432 aa
Immediate neighbours	yhfU, yhfW
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU10380

Phenotypes of a mutant

• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
• NCIB3610 hemAT mutant has higher fitness than hag mutant during pellicle formation PubMed
• NCIB3610 hemAT-hag double mutant has similar fitness to single hag mutant during pellicle formation PubMed

Database entries

• BsubCyc: BSU10380

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

• HemAT protein has a moderate oxygen affinity PubMed

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for full expression of the phoD-tatAD-tatCD operon PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): heme

• Effectors of protein activity:

• Interactions:
  • HemAT-TatAC PubMed

• Localization:
  • forms clusters at the cell poles PubMed
  • homogeneous cytoplasmic distribution in chain-forming cells during the logarithmic phase PubMed
  • polar foci in individual cells at later growth stages PubMed

Database entries

• BsubCyc: BSU10380

• Structure: 1OR4 (in cyano-liganded form), 1OR6 PubMed

• UniProt: O07621

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hemAT PubMed

• Expression browser: hemAT PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • in minimal medium, HemAT is present with 19,000 +/- 3,900 molecules per cell PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1095 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1803 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3169 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 966 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1236 PubMed

Biological materials

• Mutant:
  • TB239 (hemAT::neo in 168) PubMed
  • TB241 (hemAT::neo in NCIB3610) PubMed
  • TB244 amyE::Phy-sfgfp (hemAT::neo in NCIB3610 with constitutive expressed sfgfp) PubMed
  • TB243 amyE::Phy-mKATE2 (hemAT::neo in NCIB3610 with constitutive expressed mKATE2) PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Akos T Kovacs