MotA
| Gene name | motA |
| Synonyms | mot |
| Essential | no |
| Product | motility protein (flagellar motor rotation) |
| Function | motility and chemotaxis |
| Gene expression levels in SubtiExpress: motA | |
| Interactions involving this protein in SubtInteract: MotA | |
| MW, pI | 29 kDa, 5.01 |
| Gene length, protein length | 810 bp, 270 aa |
| Immediate neighbours | motB, clpE |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
motility and chemotaxis, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13690
Phenotypes of a mutant
- loss of swimming and swarming motility PubMed
- mucoid phenotype due to the DegU-P activated overexpression of the capB-capC-capA-capE operon and resulting overproduction of poly-gamma-glutamate PubMed
- not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
Database entries
- BsubCyc: BSU13690
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
- A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: motA family (according to Swiss-Prot)
- Paralogous protein(s): MotP
Extended information on the protein
- Kinetic information:
- Modification:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- BsubCyc: BSU13690
- Structure:
- UniProt: P28611
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor: SigD PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- 1A631 ( motA::erm), PubMed, available at BGSC
- 1A923 ( motA::erm), PubMed, available at BGSC
- DS7498 (marker-less in NCIB3610) PubMed
- TB193 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
- TB210 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Theresa Hölscher, Benjamin Bartels, Yu-Cheng Lin, Ramses Gallegos-Monterrosa, Alexa Price-Whelan, Roberto Kolter, Lars E P Dietrich, Ákos T Kovács
Motility, Chemotaxis and Aerotaxis Contribute to Competitiveness during Bacterial Pellicle Biofilm Development.
J Mol Biol: 2015, 427(23);3695-3708
[PubMed:26122431]
[WorldCat.org]
[DOI]
(I p)
Yuka Takahashi, Masahiro Ito
Mutational analysis of charged residues in the cytoplasmic loops of MotA and MotP in the Bacillus subtilis flagellar motor.
J Biochem: 2014, 156(4);211-20
[PubMed:24771657]
[WorldCat.org]
[DOI]
(I p)
Jia Mun Chan, Sarah B Guttenplan, Daniel B Kearns
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
J Bacteriol: 2014, 196(4);740-53
[PubMed:24296669]
[WorldCat.org]
[DOI]
(I p)
Yun Chen, Yunrong Chai, Jian-hua Guo, Richard Losick
Evidence for cyclic Di-GMP-mediated signaling in Bacillus subtilis.
J Bacteriol: 2012, 194(18);5080-90
[PubMed:22821967]
[WorldCat.org]
[DOI]
(I p)
Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766]
[WorldCat.org]
[DOI]
(I p)
M Z Gilman, M J Chamberlin
Developmental and genetic regulation of Bacillus subtilis genes transcribed by sigma 28-RNA polymerase.
Cell: 1983, 35(1);285-93
[PubMed:6313226]
[WorldCat.org]
[DOI]
(P p)
