YabT
- Description: Ser/Thr kinase, controls translation and DNA integrity during spore development
| Gene name | yabT |
| Synonyms | |
| Essential | no |
| Product | Ser/Thr kinase |
| Function | control of DNA integrity during spore development |
| Gene expression levels in SubtiExpress: yabT | |
| Interactions involving this protein in SubtInteract: YabT | |
| MW, pI | 37 kDa, 9.943 |
| Gene length, protein length | 1014 bp, 338 aa |
| Immediate neighbours | yabS, tilS |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
protein modification, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00660
Phenotypes of a mutant
- increased sensitivity to DNA damage during spore development PubMed
Database entries
- BsubCyc: BSU00660
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- phosphorylation of RecA on Ser-2 PubMed
- phosphorylation of tufA on Thr-63 during sporulation PubMed
- Protein family: protein kinase domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- autophosphorylation is stimulated by non-specific binding to DNA PubMed
- Localization:
- colocalizes strongly with the septal membrane separating the mother cells from the forespore PubMed
Database entries
- BsubCyc: BSU00660
- Structure:
- UniProt: P37562
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP577 (erm), available in Jörg Stülke's lab
- Expression vector:
- purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Jörg Stülke's lab
- purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Jörg Stülke's lab
- purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Jörg Stülke's lab
- lacZ fusion:
- translational lacZ fusion (in pAC7): pGP831, available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Sandro F F Pereira, Ruben L Gonzalez, Jonathan Dworkin
Protein synthesis during cellular quiescence is inhibited by phosphorylation of a translational elongation factor.
Proc Natl Acad Sci U S A: 2015, 112(25);E3274-81
[PubMed:26056311]
[WorldCat.org]
[DOI]
(I p)
Lei Shi, Nathalie Pigeonneau, Vaishnavi Ravikumar, Paula Dobrinic, Boris Macek, Damjan Franjevic, Marie-Francoise Noirot-Gros, Ivan Mijakovic
Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues.
Front Microbiol: 2014, 5;495
[PubMed:25278935]
[WorldCat.org]
[DOI]
(P e)
Ahasanul Kobir, Sandrine Poncet, Vladimir Bidnenko, Olivier Delumeau, Carsten Jers, Samira Zouhir, Rosa Grenha, Sylvie Nessler, Phillipe Noirot, Ivan Mijakovic
Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties.
Mol Microbiol: 2014, 92(5);1129-41
[PubMed:24731262]
[WorldCat.org]
[DOI]
(I p)
Vladimir Bidnenko, Lei Shi, Ahasanul Kobir, Magali Ventroux, Nathalie Pigeonneau, Céline Henry, Alain Trubuil, Marie-Françoise Noirot-Gros, Ivan Mijakovic
Bacillus subtilis serine/threonine protein kinase YabT is involved in spore development via phosphorylation of a bacterial recombinase.
Mol Microbiol: 2013, 88(5);921-35
[PubMed:23634894]
[WorldCat.org]
[DOI]
(I p)
Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325]
[WorldCat.org]
[DOI]
(P p)
