CwlT

• Description: cell wall hydrolase, required for conjugation of ICEBs1, part of the type IV secretion system for DNA transfer , C-terminal domain hydrolyzes bond between D-Glu and m-DAP
  
  

Gene name	cwlT
Synonyms	yddH
Essential
Product	cell wall hydrolase
Function	conjugative transfer of ICEBs1
Gene expression levels in SubtiExpress: cwlT
MW, pI	36 kDa, 8.632
Gene length, protein length	987 bp, 329 aa
Immediate neighbours	yddG, yddI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

mobile genetic elements, cell wall degradation/ turnover

The gene

Basic information

• Locus tag: BSU04970

Database entries

• BsubCyc: BSU04970

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D-γ-glutamate and meso-diaminopimelic acid) PubMed
• Protein family: nlpC/p60 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine PubMed
  • C-terminal endopeptidase domain cleaves the bond between D-γ-glutamate and meso-diaminopimelic acid PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (with signal peptide) PubMed
  • may be a lipoprotein, but the lipid anchor is not required for function PubMed

Database entries

• BsubCyc: BSU04970

• Structure:

• UniProt: P96645

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: cwlT PubMed

• Sigma factor:

• Regulation:
  • induced by mitomycin C PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tyler DeWitt, Alan D Grossman
The bifunctional cell wall hydrolase CwlT is needed for conjugation of the integrative and conjugative element ICEBs1 in Bacillus subtilis and B. anthracis.
J Bacteriol: 2014, 196(8);1588-96
[PubMed:24532767] [WorldCat.org] [DOI] (I p)

Tatsuya Fukushima, Toshihiko Kitajima, Hiroyuki Yamaguchi, Qin Ouyang, Kazumi Furuhata, Hiroki Yamamoto, Toshio Shida, Junichi Sekiguchi
Identification and characterization of novel cell wall hydrolase CwlT: a two-domain autolysin exhibiting n-acetylmuramidase and DL-endopeptidase activities.
J Biol Chem: 2008, 283(17);11117-25
[PubMed:18305117] [WorldCat.org] [DOI] (P p)