FloT

• Description: similar to flotillin 1, orchestration of physiological processes in lipid microdomains, involved in the control of membrane fluidity, confers (together with YuaF) resistance to cefuroxime
  
  

Gene name	floT
Synonyms	yuaG, yuaH
Essential	no
Product	bacterial flotillin-like protein
Function	involved in the control of membrane fluidity
Gene expression levels in SubtiExpress: floT
Interactions involving this protein in SubtInteract: FloT
MW, pI	55 kDa, 5.135
Gene length, protein length	1527 bp, 509 aa
Immediate neighbours	yuaI, yuaF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biofilm formation, membrane dynamics, membrane proteins sporulation/ other, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

• Locus tag: BSU31010

Phenotypes of a mutant

• • delayed onset of sporulation, reduced sporulation frequency
  • defect in motility PubMed
  • reduced protein secretion PubMed
  • a floT floA double mutant does not induce KinC-dependent biofilm formation upon addition of surfactin PubMed
  • a floT floA double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency PubMed
  • a floT floA double mutant has a sporulation defect, due to the lack of FtsH PubMed
  • a floT mutant displays a defective growth under oxygen-limiting conditions PubMed

Database entries

• BsubCyc: BSU31010

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • SigW-dependent expression of fabF and the yuaF-floT-yuaI operon result in reduced membrane fluidity PubMed
  • recruits YuaF to focal assemblies PubMed
  • controls protease activity of FtsH PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms homo-oligomers PubMed
  • oligomerization depends on the glutamate-alanine repeats PubMed
  • YuaF-FloT PubMed
  • FloT-FtsH PubMed
  • FloT-FloA PubMed
  • FloT-SecY PubMed
  • FloT-FtsX PubMed
  • FloT-OppA PubMed
  • FloT-SdhA PubMed
  • FloT-ResE PubMed

• Localization:
  • membrane associated PubMed
  • co-localizes with FloA and KinC in discrete foci membrane PubMed
  • forms discrete focal structures in the cytoplasma membrane PubMed
  • 6 foci FloT of are detected in strain 3610 PubMed

Database entries

• BsubCyc: BSU31010

• Structure:

• UniProt: O32076

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yuaF-floT-yuaI PubMed

• Expression browser: floT PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • expressed upon cell wall stress (SigW) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1215 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 236 PubMed

Biological materials

• Mutant:
  • JS152 (markerless), available in Daniel Lopez's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:
  • GK38 (168 amyE::PfloT-yfp (spc)), available in Daniel Lopez's lab
  • JS280 (3610 amyE::floT-gfp(spc)), available in Daniel Lopez's lab
  • JS153 (3610 lacA::floT-mEos2 (mls)), available in Daniel Lopez's lab
  • JS166 (3610 lacA::floT-PAmCherry (mls)), available in Daniel Lopez's lab

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH) for FloT PubMed, available in Daniel Lopez's lab

• Antibody:

References

Reviews

Marc Bramkamp, Daniel Lopez
Exploring the existence of lipid rafts in bacteria.
Microbiol Mol Biol Rev: 2015, 79(1);81-100
[PubMed:25652542] [WorldCat.org] [DOI] (I p)