Smc

• Description: part of the condensin complex, chromosomal origin condensation and segregation SMC protein
  
  

Gene name	smc
Synonyms	ylqA
Essential	yes PubMed
Product	chromosome condensation and segregation SMC protein
Function	segregation of replication origins
Gene expression levels in SubtiExpress: smc
Interactions involving this protein in SubtInteract: Smc
Metabolic function and regulation of this protein in SubtiPathways: Smc
MW, pI	135 kDa, 5.266
Gene length, protein length	3558 bp, 1186 aa
Immediate neighbours	rnc, ftsY
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, essential genes

The gene

Basic information

• Locus tag: BSU15940

Phenotypes of a mutant

• essential PubMed
• smc mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth PubMed

Database entries

• BsubCyc: BSU15940

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: SMC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • ATP-induced DNA-binding results in a more open conformation of the Smc dimer, and thus of the condension complex PubMed

• Interactions:
  • part of the condensin complex PubMed
  • forms dimers PubMed
  • ScpA-Smc PubMed
  • ScpB-Smc, PubMed, this interaction takes place only in the presence of ScpA PubMed
  • Smc is a member of a suspected group of hubs proteins that were suggested to be involved in a large number of interactions PubMed

• Localization:
  • nucleoid (heterogeneous) PubMed
  • the condensin (Smc)2-ScpA-ScpB complex forms two bipolar assemblies on the chromosome, one in each cell half, localization depends on Spo0J PubMed

Database entries

• BsubCyc: BSU15940

• Structure:
  • 3ZGX (complex between the head domain of Smc (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of ScpA) PubMed

• UniProt: P51834

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rnc-smc-ftsY PubMed

• Expression browser: smc PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A822 ( smc::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Sophie Nolivos, David Sherratt
The bacterial chromosome: architecture and action of bacterial SMC and SMC-like complexes.
FEMS Microbiol Rev: 2014, 38(3);380-92
[PubMed:24118085] [WorldCat.org] [DOI] (I p)

Rodrigo Reyes-Lamothe, Emilien Nicolas, David J Sherratt
Chromosome replication and segregation in bacteria.
Annu Rev Genet: 2012, 46;121-43
[PubMed:22934648] [WorldCat.org] [DOI] (I p)

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Christine Kaimer, Peter L Graumann
Players between the worlds: multifunctional DNA translocases.
Curr Opin Microbiol: 2011, 14(6);719-25
[PubMed:22047950] [WorldCat.org] [DOI] (I p)

Peter L Graumann, Tobias Knust
Dynamics of the bacterial SMC complex and SMC-like proteins involved in DNA repair.
Chromosome Res: 2009, 17(2);265-75
[PubMed:19308706] [WorldCat.org] [DOI] (I p)