GdpP

• Description: cyclic di-AMP phosphodiesterase

Gene name	gdpP
Synonyms	yybT
Essential	no
Product	c-di-AMP phosphodiesterase
Function	control of sporulation initiation, cell wall homeostasis
Gene expression levels in SubtiExpress: gdpP
Metabolic function and regulation of this protein in SubtiPathways: GdpP
MW, pI	74 kDa, 4.865
Gene length, protein length	1977 bp, 659 aa
Immediate neighbours	rplI, yybS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides, membrane proteins

The gene

Basic information

• Locus tag: BSU40510

Phenotypes of a mutant

• inactivation of gdpP strongly restores beta-lactam resistance in a sigM mutant PubMed

Database entries

• BsubCyc: BSU40510

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • cyclic dinucleotide phosphodiesterase, hydrolysis of di-cyclic AMP PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • two transmembrane domains at the N-terminus PubMed
  • a domain that shares minimum sequence homology with Heme-binding Per-ARNT-Sim (PAS) domains (80 aa) PubMed
  • degenerate GGDEF domain (aa 173 - 301) PubMed
  • DHH/ DHHA1 phosphodiesterase domain PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:
  • ppGpp inhibits cyclic dinucleotide phosphodiesterase activity PubMed
  • Heme binding suppresses phosphodiesterase activity PubMed

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU40510

• Structure:
  • 2M1C (N-terminal PAS domain of GdpP from Geobacillus thermodenitrificans) PubMed

• UniProt: P37484

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yybS-gdpP-rplI (according to DBTBS)

• Expression browser: gdpP PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • there is an internal SigD-dependent antisense transcript (as-gdpP) PubMed

Biological materials

• Mutant:
  • GP998 (gdpP::spc), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Fabian M Commichau, Achim Dickmanns, Jan Gundlach, Ralf Ficner, Jörg Stülke
A jack of all trades: the multiple roles of the unique essential second messenger cyclic di-AMP.
Mol Microbiol: 2015, 97(2);189-204
[PubMed:25869574] [WorldCat.org] [DOI] (I p)

GdpP homologs in other organisms

Linda M Holland, Sinéad T O'Donnell, Dmitri A Ryjenkov, Larissa Gomelsky, Shawn R Slater, Paul D Fey, Mark Gomelsky, James P O'Gara
A staphylococcal GGDEF domain protein regulates biofilm formation independently of cyclic dimeric GMP.
J Bacteriol: 2008, 190(15);5178-89
[PubMed:18502872] [WorldCat.org] [DOI] (I p)