CsfB

• Description: forespore-specific protein, inhibitor of SigG and of SigE
  
  

Gene name	csfB
Synonyms	gin, yaaM
Essential	no
Product	inhibitor of SigG and SigE
Function	control of SigG and SigE activity
Gene expression levels in SubtiExpress: csfB
Interactions involving this protein in SubtInteract: CsfB
MW, pI	7 kDa, 7.671
Gene length, protein length	192 bp, 64 aa
Immediate neighbours	rrnA-5S, xpaC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, sporulation proteins

This gene is a member of the following regulons

SigF regulon, SigK regulon

The gene

Basic information

• Locus tag: BSU00240

Phenotypes of a mutant

• premature activation of SigG PubMed

Database entries

• BsubCyc: BSU00240

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: inhibitor of SigG PubMed1 PubMed2

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CsfB-SigG to inhibit SigG activity PubMed
  • CsfB-SigE to inhibit SigE activity PubMed

• Localization: forespore and mother cell PubMed

Database entries

• BsubCyc: BSU00240

• Structure:

• UniProt: P37534

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: csfB PubMed

• Expression browser: csfB PubMed

• Sigma factor:
  • SigF PubMed, SigK PubMed

• Regulation:
  • expressed early during sporulation in the forespore (SigF) PubMed
  • expressed after completion of forespore engulfment in the mother cell (SigK) PubMed
• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Mónica Serrano, JinXin Gao, João Bota, Ashley R Bate, Jeffrey Meisner, Patrick Eichenberger, Charles P Moran, Adriano O Henriques
Dual-specificity anti-sigma factor reinforces control of cell-type specific gene expression in Bacillus subtilis.
PLoS Genet: 2015, 11(4);e1005104
[PubMed:25835496] [WorldCat.org] [DOI] (I e)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Mónica Serrano, Gonçalo Real, Joana Santos, Jorge Carneiro, Charles P Moran, Adriano O Henriques
A negative feedback loop that limits the ectopic activation of a cell type-specific sporulation sigma factor of Bacillus subtilis.
PLoS Genet: 2011, 7(9);e1002220
[PubMed:21935351] [WorldCat.org] [DOI] (I p)

Vasant K Chary, Panagiotis Xenopoulos, Avigdor Eldar, Patrick J Piggot
Loss of compartmentalization of σ(E) activity need not prevent formation of spores by Bacillus subtilis.
J Bacteriol: 2010, 192(21);5616-24
[PubMed:20802044] [WorldCat.org] [DOI] (I p)

Lamya Rhayat, Sandra Duperrier, Rut Carballido-López, Olivier Pellegrini, Patrick Stragier
Genetic dissection of an inhibitor of the sporulation sigma factor sigma(G).
J Mol Biol: 2009, 390(5);835-44
[PubMed:19497328] [WorldCat.org] [DOI] (I p)

Céline Karmazyn-Campelli, Lamya Rhayat, Rut Carballido-López, Sandra Duperrier, Niels Frandsen, Patrick Stragier
How the early sporulation sigma factor sigmaF delays the switch to late development in Bacillus subtilis.
Mol Microbiol: 2008, 67(5);1169-80
[PubMed:18208527] [WorldCat.org] [DOI] (I p)

Vasant K Chary, Panagiotis Xenopoulos, Patrick J Piggot
Expression of the sigmaF-directed csfB locus prevents premature appearance of sigmaG activity during sporulation of Bacillus subtilis.
J Bacteriol: 2007, 189(23);8754-7
[PubMed:17921305] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

A Decatur, R Losick
Identification of additional genes under the control of the transcription factor sigma F of Bacillus subtilis.
J Bacteriol: 1996, 178(16);5039-41
[PubMed:8759874] [WorldCat.org] [DOI] (P p)

C D Webb, A Decatur, A Teleman, R Losick
Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis.
J Bacteriol: 1995, 177(20);5906-11
[PubMed:7592342] [WorldCat.org] [DOI] (P p)