TrmU

• Description: tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase, involved in 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules
  
  

Gene name	trmU
Synonyms	yrrA
Essential	yes PubMed
Product	tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase
Function	tRNA modification
Gene expression levels in SubtiExpress: trmU
MW, pI	42 kDa, 4.729
Gene length, protein length	1122 bp, 374 aa
Immediate neighbours	yrrB, yrvO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, membrane proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU27500

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU27500

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with YrvO) PubMed

• Protein family: mnmA/TRMU family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU27500

• Structure:

• UniProt: O35020

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cymR-yrvO-trmU PubMed

• Expression browser: trmU PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 164 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 304 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Katherine A Black, Patricia C Dos Santos
Abbreviated Pathway for Biosynthesis of 2-Thiouridine in Bacillus subtilis.
J Bacteriol: 2015, 197(11);1952-62
[PubMed:25825430] [WorldCat.org] [DOI] (I p)

Soon-Yong Choi, Dindo Reyes, Montira Leelakriangsak, Peter Zuber
The global regulator Spx functions in the control of organosulfur metabolism in Bacillus subtilis.
J Bacteriol: 2006, 188(16);5741-51
[PubMed:16885442] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)