MutM

• Description: formamidopyrimidine-DNA glycosidase
  
  

Gene name	mutM
Synonyms	ytaE
Essential	no
Product	formamidopyrimidine-DNA glycosidase
Function	DNA repair
Gene expression levels in SubtiExpress: mutM
MW, pI	31 kDa, 8.889
Gene length, protein length	834 bp, 278 aa
Immediate neighbours	ytaF, polA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU29080

Phenotypes of a mutant

• increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage PubMed
• absence of MutM promotes mutagenesis allowing nutritionally stressed B. subtilis cells to escape from growth limiting conditions PubMed

Database entries

• BsubCyc: BSU29080

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine (according to Swiss-Prot)

• Protein family: FPG family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU29080

• Structure: 2F5S (complex with oxoG:C, Geobacillus stearothermophilus)

• UniProt: O34403

• KEGG entry: [2]

• E.C. number: 3.2.2.23

Expression and regulation

• Operon:

• Expression browser: mutM PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

Martha Gómez-Marroquín, Luz E Vidales, Bernardo N Debora, Fernando Santos-Escobar, Armando Obregón-Herrera, Eduardo A Robleto, Mario Pedraza-Reyes
Role of Bacillus subtilis DNA Glycosylase MutM in Counteracting Oxidatively Induced DNA Damage and in Stationary-Phase-Associated Mutagenesis.
J Bacteriol: 2015, 197(11);1963-71
[PubMed:25825434] [WorldCat.org] [DOI] (I p)

Fernando Santos-Escobar, J Félix Gutiérrez-Corona, Mario Pedraza-Reyes
Role of Bacillus subtilis error prevention oxidized guanine system in counteracting hexavalent chromium-promoted oxidative DNA damage.
Appl Environ Microbiol: 2014, 80(17);5493-502
[PubMed:24973075] [WorldCat.org] [DOI] (I p)

Yan Qi, Marie C Spong, Kwangho Nam, Martin Karplus, Gregory L Verdine
Entrapment and structure of an extrahelical guanine attempting to enter the active site of a bacterial DNA glycosylase, MutM.
J Biol Chem: 2010, 285(2);1468-78
[PubMed:19889642] [WorldCat.org] [DOI] (I p)

Luz E Vidales, Lluvia C Cárdenas, Eduardo Robleto, Ronald E Yasbin, Mario Pedraza-Reyes
Defects in the error prevention oxidized guanine system potentiate stationary-phase mutagenesis in Bacillus subtilis.
J Bacteriol: 2009, 191(2);506-13
[PubMed:19011023] [WorldCat.org] [DOI] (I p)