YsxC

• Description: assembly of the 50S subunit of the ribosome
  
  

Gene name	ysxC
Synonyms	orfX
Essential	yes PubMed
Product	GTPase
Function	ribosome assembly
Gene expression levels in SubtiExpress: ysxC
Interactions involving this protein in SubtInteract: YsxC
Metabolic function and regulation of this protein in SubtiPathways: ysxC
MW, pI	21 kDa, 9.708
Gene length, protein length	585 bp, 195 aa
Immediate neighbours	ysxD, lonA
Sequences	Protein DNA DNA_with_flanks
Genetic context
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, membrane proteins, GTP-binding proteins

This gene is a member of the following regulons

CtsR regulon

The gene

Basic information

• Locus tag: BSU28190

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU28190

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP

• Protein family: engB family (according to Swiss-Prot) Era/Obg family

• Paralogous protein(s): Era, Obg, YphC, YsxC, RbgA, YqeH, ThdF, YyaF, YnbA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YsxC-RplL PubMed
  • YsxC-RplF PubMed
  • YsxC-RplC PubMed
  • YsxC-RplA PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU28190

• Structure: 1SVI (complex with GDP), 1SUL

• UniProt: P38424

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lonA-ysxC PubMed

• Expression browser: ysxC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by heat stress (CtsR) PubMed

• Regulatory mechanism:
  • CtsR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 114 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 113 PubMed

Biological materials

• Mutant:

• Expression vector:
  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP834, available in Stülke lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP842, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

References

Reviews

Natalie Verstraeten, Maarten Fauvart, Wim Versées, Jan Michiels
The universally conserved prokaryotic GTPases.
Microbiol Mol Biol Rev: 2011, 75(3);507-42, second and third pages of table of contents
[PubMed:21885683] [WorldCat.org] [DOI] (I p)

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)