AccC

• Description: acetyl-CoA carboxylase (biotin carboxylase subunit)
  
  

Gene name	accC
Synonyms	yqhX
Essential	yes PubMed
Product	acetyl-CoA carboxylase (biotin carboxylase subunit)
Function	production of malonyl-CoA, the substrate for fatty acid biosynthesis
Gene expression levels in SubtiExpress: accC
Interactions involving this protein in SubtInteract: AccC
Metabolic function and regulation of this protein in SubtiPathways: accC
MW, pI	49 kDa, 4.72
Gene length, protein length	1350 bp, 450 aa
Immediate neighbours	yqhY, accB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, essential genes

The gene

Basic information

• Locus tag: BSU24340

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU24340

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + biotin-carboxyl-carrier protein + CO₂ = ADP + phosphate + carboxybiotin-carboxyl-carrier protein (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s): YotE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (AccA-AccD)-AccB-AccC (acetyl-CoA carboxylase complex)

• Localization:

Database entries

• BsubCyc: BSU24340

• Structure: 1BNC (the protein from E. coli) PubMed

• UniProt: P49787

• KEGG entry: [3]

• E.C. number: 6.3.4.14, 6.4.1.2

Expression and regulation

• Operon: accB-accC-yqhY PubMed

• Expression browser: accC PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 527 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 286 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 634 PubMed

Biological materials

• Mutant:
  • GP1478 (chromosomal accC-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• FLAG-tag construct:
  • GP1483 (spc, based on pGP1331), available in Jörg Stülke's lab

• Antibody:

References

Reviews

L Tong
Acetyl-coenzyme A carboxylase: crucial metabolic enzyme and attractive target for drug discovery.
Cell Mol Life Sci: 2005, 62(16);1784-803
[PubMed:15968460] [WorldCat.org] [DOI] (P p)

Stephen W White, Jie Zheng, Yong-Mei Zhang, Rock
The structural biology of type II fatty acid biosynthesis.
Annu Rev Biochem: 2005, 74;791-831
[PubMed:15952903] [WorldCat.org] [DOI] (P p)

John E Cronan, Grover L Waldrop
Multi-subunit acetyl-CoA carboxylases.
Prog Lipid Res: 2002, 41(5);407-35
[PubMed:12121720] [WorldCat.org] [DOI] (P p)

Original Publications

P Marini, S J Li, D Gardiol, J E Cronan, D de Mendoza
The genes encoding the biotin carboxyl carrier protein and biotin carboxylase subunits of Bacillus subtilis acetyl coenzyme A carboxylase, the first enzyme of fatty acid synthesis.
J Bacteriol: 1995, 177(23);7003-6
[PubMed:7592499] [WorldCat.org] [DOI] (P p)

G L Waldrop, I Rayment, H M Holden
Three-dimensional structure of the biotin carboxylase subunit of acetyl-CoA carboxylase.
Biochemistry: 1994, 33(34);10249-56
[PubMed:7915138] [WorldCat.org] [DOI] (P p)