TyrZ

• Description: alternative tyrosyl-tRNA synthetase, required to prevent misincorporation of D-Tyr into proteins
  
  

Gene name	tyrZ
Synonyms	ipa-9r, tyrS1, tyrT
Essential	no
Product	tyrosyl-tRNA synthetase (minor)
Function	translation
Gene expression levels in SubtiExpress: tyrZ
Metabolic function and regulation of this protein in SubtiPathways: tyrZ
MW, pI	46 kDa, 6.661
Gene length, protein length	1239 bp, 413 aa
Immediate neighbours	ywaE, ywaD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

DtrR regulon, T-box

The gene

Basic information

• Locus tag: BSU38460

Phenotypes of a mutant

• sensitive against D-Tyr PubMed
• a tyrS tyrZ double mutant is not viable PubMed

Database entries

• BsubCyc: BSU38460

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot) ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot)
  • TyrZ is highly specific for L-Tyr, prevents misincorporation of D-Tyr into proteins, less processive than TyrS PubMed

• Protein family: TyrS type 2 subfamily (according to Swiss-Prot), class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on Thr-311 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU38460

• Structure:

• UniProt: P25151

• KEGG entry: [3]

• E.C. number: 6.1.1.1

Expression and regulation

• Operon: dtrR-tyrZ PubMed

• Expression browser: tyrZ PubMed

• Sigma factor: tyrZ PubMed

• Regulation:
  • induced by tyrosine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed
  • DtrR: transcriptional repression PubMed

• Additional information:

Biological materials

• Mutant: QB53532 (aphA3), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: