FliM
- Description: flagellar motor switch protein, part of the basal body C-ring
| Gene name | fliM |
| Synonyms | |
| Essential | no |
| Product | flagellar motor switch protein |
| Function | movement and chemotaxis |
| Gene expression levels in SubtiExpress: fliM | |
| MW, pI | 37 kDa, 4.691 |
| Gene length, protein length | 996 bp, 332 aa |
| Immediate neighbours | fliL, fliY |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
motility and chemotaxis, membrane proteins
This gene is a member of the following regulons
CodY regulon, DegU regulon, SigD regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU16310
Phenotypes of a mutant
Database entries
- BsubCyc: BSU16310
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: fliM family (according to Swiss-Prot)
- Paralogous protein(s): FliY
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU16310
- Structure:
- UniProt: P23453
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: part of the fla-che operon (see there for regulation)
- Regulation: see fla-che operon
- Regulatory mechanism: see fla-che operon
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 127 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Jan S Schuhmacher, Florian Rossmann, Felix Dempwolff, Carina Knauer, Florian Altegoer, Wieland Steinchen, Anja K Dörrich, Andreas Klingl, Milena Stephan, Uwe Linne, Kai M Thormann, Gert Bange
MinD-like ATPase FlhG effects location and number of bacterial flagella during C-ring assembly.
Proc Natl Acad Sci U S A: 2015, 112(10);3092-7
[PubMed:25733861]
[WorldCat.org]
[DOI]
(I p)
Serena Mordini, Cecilia Osera, Simone Marini, Francesco Scavone, Riccardo Bellazzi, Alessandro Galizzi, Cinzia Calvio
The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis.
PLoS One: 2013, 8(12);e85065
[PubMed:24386445]
[WorldCat.org]
[DOI]
(I e)
Sarah B Guttenplan, Sidney Shaw, Daniel B Kearns
The cell biology of peritrichous flagella in Bacillus subtilis.
Mol Microbiol: 2013, 87(1);211-29
[PubMed:23190039]
[WorldCat.org]
[DOI]
(I p)
Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253]
[WorldCat.org]
[DOI]
(P p)
H Werhane, P Lopez, M Mendel, M Zimmer, G W Ordal, L M Márquez-Magaña
The last gene of the fla/che operon in Bacillus subtilis, ylxL, is required for maximal sigmaD function.
J Bacteriol: 2004, 186(12);4025-9
[PubMed:15175317]
[WorldCat.org]
[DOI]
(P p)
Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647]
[WorldCat.org]
[DOI]
(P p)
W Estacio, S S Anna-Arriola, M Adedipe, L M Márquez-Magaña
Dual promoters are responsible for transcription initiation of the fla/che operon in Bacillus subtilis.
J Bacteriol: 1998, 180(14);3548-55
[PubMed:9657996]
[WorldCat.org]
[DOI]
(P p)
L M Márquez-Magaña, M J Chamberlin
Characterization of the sigD transcription unit of Bacillus subtilis.
J Bacteriol: 1994, 176(8);2427-34
[PubMed:8157612]
[WorldCat.org]
[DOI]
(P p)
A R Zuberi, D S Bischoff, G W Ordal
Nucleotide sequence and characterization of a Bacillus subtilis gene encoding a flagellar switch protein.
J Bacteriol: 1991, 173(2);710-9
[PubMed:1898932]
[WorldCat.org]
[DOI]
(P p)
