SasB

• Description: (p)ppGpp synthetase
  
  

Gene name	sasB
Synonyms	yjbM
Essential	no
Product	(p)ppGpp synthetase
Function	ppGpp synthesis independent from stringent response
Gene expression levels in SubtiExpress: sasB
MW, pI	24 kDa, 6.708
Gene length, protein length	633 bp, 211 aa
Immediate neighbours	yjbL, nadF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides

The gene

Basic information

• Locus tag: BSU11600

Phenotypes of a mutant

• a relA sasA sasB triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of guaB or inactivation of codY PubMed
• a relA sasA sasB triple mutant acquires suppressor mutations in guaA, guaB, gmk or inactivation of codY PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): SasA

Extended information on the protein

• Kinetic information:

• Domains: single ppGpp synthetase domain PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31611

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yjbL-sasB-nadF-yjbO PubMed

• Expression browser: sasB PubMed

• Sigma factor:

• Regulation: expressed during exponential growth

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 169 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: