FlhA

• Description: part of the flagellar type III export apparatus
  
  

Gene name	flhA
Synonyms
Essential	no
Product	part of the flagellar type III export apparatus
Function	export of flagellum components
Gene expression levels in SubtiExpress: flhA
Interactions involving this protein in SubtInteract: FlhA
MW, pI	73 kDa, 4.637
Gene length, protein length	2031 bp, 677 aa
Immediate neighbours	flhB, flhF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, protein secretion, membrane proteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16390

Phenotypes of a mutant

• no secretion of FlgM, permanent inhibition of SigD PubMed

Database entries

• BsubCyc: BSU16390

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: FHIPEP (flagella/HR/invasion proteins export pore) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • FlhA-FliJ PubMed
  • FlhA-FliD PubMed
  • FlhA-Hag PubMed
  • FlhA-FliT PubMed
  • FlhA-(FliD-FliT) PubMed
  • FlhA-(FliS-Hag) PubMed

• Localization: cell membrane PubMed

Database entries

• BsubCyc: BSU16390

• Structure: 3MIX (cytosolic domain) PubMed

• UniProt: P35620

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: flhA PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed
  • DegU-P: transcription repression PubMed
  • DegU-P in complex with SwrA: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 167 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: