YjbH

• Description: adaptor protein for ClpX-ClpP-catalyzed Spx degradation, confers resistance against nitrosating agents
  
  

Gene name	yjbH
Synonyms
Essential	no
Product	adaptor protein
Function	stimulation of Spx degradation
Gene expression levels in SubtiExpress: yjbH
Interactions involving this protein in SubtInteract: YjbH
MW, pI	31 kDa, 5.206
Gene length, protein length	825 bp, 275 aa
Immediate neighbours	yizD, yjbI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis, resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

The gene

Basic information

• Locus tag: BSU11550

Phenotypes of a mutant

• increased thermotolerance due to increased stabiliy of Spx and thus increased expression of trxA PubMed

Database entries

• BsubCyc: BSU11550

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: adaptor protein for ClpX-ClpP-catalyzed Spx degradation PubMed

• Protein family: UPF0413 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:
  • contains Zn atoms (coordinated by the N-terminal His-rich region) PubMed

• Effectors of protein activity:
  • YjbH aggregates under stress conditions, resulting in the inability to bind to Spx, and therefore in stabilization of Spx PubMed
  • Zn atom is released upon treatment with strong oxidants PubMed
  • interaction with YirB inhibits the formation of a complex with Spx PubMed

• Interactions:
  • YjbH-Spx PubMed
  • YirB-YjbH PubMed

• Localization: cytosolic protein

Database entries

• BsubCyc: BSU11550

• Structure:

• UniProt: O31606

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yjbI-yjbH PubMed

• Expression browser: yjbH PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between yizD and yjbH PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Zuber, Oregon Health and Science University, USA Homepage

Claes von Wachenfeldt, Lund University, Sweden Homepage

References

Reviews

Noël Molière, Kürşad Turgay
General and regulatory proteolysis in Bacillus subtilis.
Subcell Biochem: 2013, 66;73-103
[PubMed:23479438] [WorldCat.org] [DOI] (P p)

Aurelia Battesti, Susan Gottesman
Roles of adaptor proteins in regulation of bacterial proteolysis.
Curr Opin Microbiol: 2013, 16(2);140-7
[PubMed:23375660] [WorldCat.org] [DOI] (I p)

Janine Kirstein, Noël Molière, David A Dougan, Kürşad Turgay
Adapting the machine: adaptor proteins for Hsp100/Clp and AAA+ proteases.
Nat Rev Microbiol: 2009, 7(8);589-99
[PubMed:19609260] [WorldCat.org] [DOI] (I p)

Original Publications

Jakob Engman, Claes von Wachenfeldt
Regulated protein aggregation: a mechanism to control the activity of the ClpXP adaptor protein YjbH.
Mol Microbiol: 2015, 95(1);51-63
[PubMed:25353645] [WorldCat.org] [DOI] (I p)

Chio Mui Chan, Erik Hahn, Peter Zuber
Adaptor bypass mutations of Bacillus subtilis spx suggest a mechanism for YjbH-enhanced proteolysis of the regulator Spx by ClpXP.
Mol Microbiol: 2014, 93(3);426-38
[PubMed:24942655] [WorldCat.org] [DOI] (I p)

Stephanie Runde, Noël Molière, Anja Heinz, Etienne Maisonneuve, Armgard Janczikowski, Alexander K W Elsholz, Ulf Gerth, Michael Hecker, Kürşad Turgay
The role of thiol oxidative stress response in heat-induced protein aggregate formation during thermotolerance in Bacillus subtilis.
Mol Microbiol: 2014, 91(5);1036-52
[PubMed:24417481] [WorldCat.org] [DOI] (I p)

Nadine Göhring, Iris Fedtke, Guoqing Xia, Ana M Jorge, Mariana G Pinho, Ute Bertsche, Andreas Peschel
New role of the disulfide stress effector YjbH in β-lactam susceptibility of Staphylococcus aureus.
Antimicrob Agents Chemother: 2011, 55(12);5452-8
[PubMed:21947404] [WorldCat.org] [DOI] (I p)

Sushma Kommineni, Saurabh K Garg, Chio Mui Chan, Peter Zuber
YjbH-enhanced proteolysis of Spx by ClpXP in Bacillus subtilis is inhibited by the small protein YirB (YuzO).
J Bacteriol: 2011, 193(9);2133-40
[PubMed:21378193] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Saurabh K Garg, Sushma Kommineni, Luke Henslee, Ying Zhang, Peter Zuber
The YjbH protein of Bacillus subtilis enhances ClpXP-catalyzed proteolysis of Spx.
J Bacteriol: 2009, 191(4);1268-77
[PubMed:19074380] [WorldCat.org] [DOI] (I p)

Jonas T Larsson, Annika Rogstam, Claes von Wachenfeldt
YjbH is a novel negative effector of the disulphide stress regulator, Spx, in Bacillus subtilis.
Mol Microbiol: 2007, 66(3);669-84
[PubMed:17908206] [WorldCat.org] [DOI] (P p)

Annika Rogstam, Jonas T Larsson, Peter Kjelgaard, Claes von Wachenfeldt
Mechanisms of adaptation to nitrosative stress in Bacillus subtilis.
J Bacteriol: 2007, 189(8);3063-71
[PubMed:17293416] [WorldCat.org] [DOI] (P p)