PcrA

• Description: ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer
  
  

Gene name	pcrA
Synonyms	yerF
Essential	yes PubMed
Product	ATP-dependent DNA helicase
Function	plasmid rolling-circle replication, conjugative transfer of ICEBs1
Gene expression levels in SubtiExpress: pcrA
Interactions involving this protein in SubtInteract: PcrA
MW, pI	83 kDa, 5.767
Gene length, protein length	2217 bp, 739 aa
Immediate neighbours	pcrB, ligA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, mobile genetic elements, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU06610

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU06610

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop PubMed
  • dismantles RecA filaments PubMed

• Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)

• Paralogous protein(s): YjcD

Extended information on the protein

• Kinetic information:

• Domains:
  • two RecA-like domains PubMed
  • intrinxically disordered C-terminal domain (for interaction with RNA polymerase) PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • unwinding of the double-stranded ICEBs1 DNA is stimulated by HelP PubMed
  • helicase activity is stimulated by interaction with RNA polymerase PubMed

• Interactions:
  • the C-terminal domain of PcrA interacts with the RNA polymerase PubMed
  • YxaL-PcrA PubMed
  • YerB-PcrA PubMed
  • YwhK-PcrA PubMed
  • UvrB-PcrA PubMed
  • YwqA-PcrA PubMed

• Localization:

Database entries

• BsubCyc: BSU06610

• Structure:
  • 2PJR (product complex, complex with sulphate ion, Geobacillus stearothermophilus)
  • 1PJR (substrate complex, complex with DNA, Geobacillus stearothermophilus)

• UniProt: O34580

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pcrB-pcrA-ligA-yerH PubMed

• Expression browser: pcrA PubMed

• Sigma factor:

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 114 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 250 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

• Antibody:

References

Reviews

Elisabeth Grohmann
Autonomous plasmid-like replication of Bacillus ICEBs1: a general feature of integrative conjugative elements?
Mol Microbiol: 2010, 75(2);261-3
[PubMed:19943906] [WorldCat.org] [DOI] (I p)