SpoVID

• Description: spore coat morphogenetic protein, promotes encasement of the spore
  
  

Gene name	spoVID
Synonyms
Essential	no
Product	spore coat morphogenetic protein
Function	spore coat assembly
Gene expression levels in SubtiExpress: spoVID
Interactions involving this protein in SubtInteract: SpoVID
MW, pI	64 kDa, 3.999
Gene length, protein length	1725 bp, 575 aa
Immediate neighbours	ysxE, hemL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU28110

Database entries

• BsubCyc: BSU28110

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • SpoIVA-SpoVID PubMed
  • SpoVID-SafA PubMed
  • SpoIVA-SpoVM-SpoVID PubMed
  • SpoVID-CotE PubMed

• Localization:
  • spore coat (basement), localization depends on SpoIVA PubMed

Database entries

• BsubCyc: BSU28110

• Structure:

• UniProt: P37963

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoVID-ysxE PubMed

• Expression browser: spoVID PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A639 ( spoVID::erm), PubMed, available at BGSC
  • 1S127 ( spoVID::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Adriano Henriques, Lisbon, Portugal homepage
• Charles Moran, Emory University, NC, USA homepage

References

Reviews

Peter T McKenney, Adam Driks, Patrick Eichenberger
The Bacillus subtilis endospore: assembly and functions of the multilayered coat.
Nat Rev Microbiol: 2013, 11(1);33-44
[PubMed:23202530] [WorldCat.org] [DOI] (I p)

Original publications

Marco Plomp, Alicia Monroe Carroll, Peter Setlow, Alexander J Malkin
Architecture and assembly of the Bacillus subtilis spore coat.
PLoS One: 2014, 9(9);e108560
[PubMed:25259857] [WorldCat.org] [DOI] (I e)

Haiyan Qiao, Daniela Krajcikova, Chunyan Xing, Baoping Lu, Jinhui Hao, Xi Ke, Hongda Wang, Imrich Barak, Jilin Tang
Study of the interactions between the key spore coat morphogenetic proteins CotE and SpoVID.
J Struct Biol: 2013, 181(2);128-35
[PubMed:23178679] [WorldCat.org] [DOI] (I p)

Melissa de Francesco, Jake Z Jacobs, Filipa Nunes, Mónica Serrano, Peter T McKenney, Ming-Hsiu Chua, Adriano O Henriques, Patrick Eichenberger
Physical interaction between coat morphogenetic proteins SpoVID and CotE is necessary for spore encasement in Bacillus subtilis.
J Bacteriol: 2012, 194(18);4941-50
[PubMed:22773792] [WorldCat.org] [DOI] (I p)

Haiyan Qiao, Daniela Krajcikova, Caisheng Liu, Yongjun Li, Hongda Wang, Imrich Barak, Jilin Tang
The interactions of spore-coat morphogenetic proteins studied by single-molecule recognition force spectroscopy.
Chem Asian J: 2012, 7(4);725-31
[PubMed:22262582] [WorldCat.org] [DOI] (I p)

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Katherine H Wang, Anabela L Isidro, Lia Domingues, Haig A Eskandarian, Peter T McKenney, Kevin Drew, Paul Grabowski, Ming-Hsiu Chua, Samantha N Barry, Michelle Guan, Richard Bonneau, Adriano O Henriques, Patrick Eichenberger
The coat morphogenetic protein SpoVID is necessary for spore encasement in Bacillus subtilis.
Mol Microbiol: 2009, 74(3);634-49
[PubMed:19775244] [WorldCat.org] [DOI] (I p)

Denisa Müllerová, Daniela Krajčíková, Imrich Barák
Interactions between Bacillus subtilis early spore coat morphogenetic proteins.
FEMS Microbiol Lett: 2009, 299(1);74-85
[PubMed:19702880] [WorldCat.org] [DOI] (I p)

Teresa Costa, Anabela L Isidro, Charles P Moran, Adriano O Henriques
Interaction between coat morphogenetic proteins SafA and SpoVID.
J Bacteriol: 2006, 188(22);7731-41
[PubMed:16950916] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

A J Ozin, C S Samford, A O Henriques, C P Moran
SpoVID guides SafA to the spore coat in Bacillus subtilis.
J Bacteriol: 2001, 183(10);3041-9
[PubMed:11325931] [WorldCat.org] [DOI] (P p)

A J Ozin, A O Henriques, H Yi, C P Moran
Morphogenetic proteins SpoVID and SafA form a complex during assembly of the Bacillus subtilis spore coat.
J Bacteriol: 2000, 182(7);1828-33
[PubMed:10714986] [WorldCat.org] [DOI] (P p)

B Beall, A Driks, R Losick, C P Moran
Cloning and characterization of a gene required for assembly of the Bacillus subtilis spore coat.
J Bacteriol: 1993, 175(6);1705-16
[PubMed:8449878] [WorldCat.org] [DOI] (P p)