SpsA

• Description: nucleotide-sugar-dependent glycosyltransferase, spore coat polysaccharide synthesis
  
  

Gene name	spsA
Synonyms	ipa-63d
Essential	no
Product	nucleotide-sugar-dependent glycosyltransferase
Function	spore coat polysaccharide synthesis
Gene expression levels in SubtiExpress: spsA
MW, pI	30 kDa, 5.358
Gene length, protein length	768 bp, 256 aa
Immediate neighbours	spsB, gerQ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU37910

Database entries

• BsubCyc: BSU37910

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: glycosyltransferase 2 family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU37910

• Structure:
  • 1QGS (complex with UDP) PubMed
  • 1H7Q PubMed

• UniProt: P39621

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spsA-spsB-spsC-spsD-spsF-spsG-spsI-spsJ-spsK-spsL PubMed

• Expression browser: spsA PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

N Tarbouriech, S J Charnock, G J Davies
Three-dimensional structures of the Mn and Mg dTDP complexes of the family GT-2 glycosyltransferase SpsA: a comparison with related NDP-sugar glycosyltransferases.
J Mol Biol: 2001, 314(4);655-61
[PubMed:11733986] [WorldCat.org] [DOI] (P p)

S J Charnock, G J Davies
Structure of the nucleotide-diphospho-sugar transferase, SpsA from Bacillus subtilis, in native and nucleotide-complexed forms.
Biochemistry: 1999, 38(20);6380-5
[PubMed:10350455] [WorldCat.org] [DOI] (P p)

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)