TetB

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  • Description: multifunctional tetracycline/Na+ resistance protein

Gene name tetB
Synonyms tetA, tetA(L)
Essential no
Product multifunctional tetracycline/Na+ resistance protein
Function resistance to tetracyclin
Gene expression levels in SubtiExpress: tetB
MW, pI 49 kDa, 9.537
Gene length, protein length 1374 bp, 458 aa
Immediate neighbours yyaP, tetL
Sequences Protein DNA DNA_with_flanks
Genetic context
TetB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TetB expression.png















Categories containing this gene/protein

transporters/ other, resistance against toxins/ antibiotics, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU40770

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: TCR/tet family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Your additional remarks

References

Wannasiri Wannarat, Shiori Motoyama, Kenta Masuda, Fujio Kawamura, Takashi Inaoka
Tetracycline tolerance mediated by gene amplification in Bacillus subtilis.
Microbiology (Reading): 2014, 160(Pt 11);2474-2480
[PubMed:25169108] [WorldCat.org] [DOI] (I p)

W Wang, A A Guffanti, Y Wei, M Ito, T A Krulwich
Two types of Bacillus subtilis tetA(L) deletion strains reveal the physiological importance of TetA(L) in K(+) acquisition as well as in Na(+), alkali, and tetracycline resistance.
J Bacteriol: 2000, 182(8);2088-95
[PubMed:10735849] [WorldCat.org] [DOI] (P p)

S J Stasinopoulos, G A Farr, D H Bechhofer
Bacillus subtilis tetA(L) gene expression: evidence for regulation by translational reinitiation.
Mol Microbiol: 1998, 30(5);923-32
[PubMed:9988470] [WorldCat.org] [DOI] (P p)

J Cheng, D B Hicks, T A Krulwich
The purified Bacillus subtilis tetracycline efflux protein TetA(L) reconstitutes both tetracycline-cobalt/H+ and Na+(K+)/H+ exchange.
Proc Natl Acad Sci U S A: 1996, 93(25);14446-51
[PubMed:8962071] [WorldCat.org] [DOI] (P p)

J Cheng, A A Guffanti, W Wang, T A Krulwich, D H Bechhofer
Chromosomal tetA(L) gene of Bacillus subtilis: regulation of expression and physiology of a tetA(L) deletion strain.
J Bacteriol: 1996, 178(10);2853-60
[PubMed:8631673] [WorldCat.org] [DOI] (P p)

J Cheng, K Baldwin, A A Guffanti, T A Krulwich
Na+/H+ antiport activity conferred by Bacillus subtilis tetA(L), a 5' truncation product of tetA(L), and related plasmid genes upon Escherichia coli.
Antimicrob Agents Chemother: 1996, 40(4);852-7
[PubMed:8849239] [WorldCat.org] [DOI] (P p)

R Sakaguchi, H Amano, K Shishido
Nucleotide sequence homology of the tetracycline-resistance determinant naturally maintained in Bacillus subtilis Marburg 168 chromosome and the tetracycline-resistance gene of B. subtilis plasmid pNS1981.
Biochim Biophys Acta: 1988, 950(3);441-4
[PubMed:2844262] [WorldCat.org] [DOI] (P p)