bdbD

168

thiol-disulfide oxidoreductase, required for the formation of thiol disulfide bonds in several proteins

Locus: BSU_33480

Molecular weight: 24.76 kDa

Isoelectric point: 5.09

Protein length: 222 aa

Gene length: 669 bp

Function: oxidative folding of proteins

Product: thiol-disulfide oxidoreductase

Essential: no

Synonyms: bdbD, yvgV

Genomic Context

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List of homologs in different organisms, belongs to COG1651 (Galperin et al., 2021)

Gene

Coordinates: 3,438,065 → 3,438,733

Phenotypes of a mutant

• loss of transformability PubMed
• sensitive to osmotic shock PubMed
• several proteins are absent from the membrane proteome of a ''bdbC-bdbD'' mutant: PubMed
• the membrane proteins BglP, TcyP, SipU, LytA, and YxaI PubMed
• the cytoplasmic or membrane-associated proteins GlkX, ProA, PyrAA, PyrAB, PyrH, PyrE, PyrF, DegS, EcfA1 PubMed

The protein

Catalyzed reaction/ biological activity

• formation of thiol disulfide bonds in ComEC and ComGC (together with BdbC) PubMed

Protein family

• Thioredoxin family (according to UniProt)

Domains

• Thioredoxin domain (aa 37-220) (according to UniProt)

Cofactors

• Ca (2+) PubMed

Structure

• 3EU3 (PDB) (reduced form), 3EU4 (PDB) (oxidized form) PubMed
• O32218 (AlphaFold)

Localization

• membrane, faced to the outer side of the membrane  PubMed

Expression and Regulation

Operons

• Genes: bdbD-bdbC

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in comK regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-A033 (yvgV::erm), available at the NBRP B. subtilis, Japan
• BKE33480 (ΔbdbD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTCCGACACCTCATCGTT,  downstream forward: _UP4_AAAGAGCTGAAAGGGAAGTA
• BKK33480 (ΔbdbD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTCCGACACCTCATCGTT,  downstream forward: _UP4_AAAGAGCTGAAAGGGAAGTA

References

Reviews

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Original Publications

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