AccD

• Description: acetyl-CoA carboxylase (beta subunit)
  
  

Gene name	accD
Synonyms	yttI
Essential	yes PubMed
Product	acetyl-CoA carboxylase (beta subunit))
Function	production of malonyl-CoA, the substrate for fatty acid biosynthesis
Gene expression levels in SubtiExpress: accD
Interactions involving this protein in SubtInteract: AccD
Metabolic function and regulation of this protein in SubtiPathways: accD
MW, pI	32 kDa, 5.344
Gene length, protein length	873 bp, 290 aa
Immediate neighbours	accA, ytsJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, essential genes, phosphoproteins

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU29210

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU29210

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-205 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (AccA-AccD)-AccB-AccC (acetyl-CoA carboxylase complex)

• Localization: Membrane-proximal (Spotty) PubMed

Database entries

• BsubCyc: BSU29210

• Structure: 2F9I (from Staphylococcus aureus; equivalent to AccD-AccA, 64% identity) PubMed

• UniProt: C0SP93

• KEGG entry: [3]

• E.C. number: 6.4.1.2

Expression and regulation

• Operon: accD-accA PubMed

• Expression browser: accD PubMed

• Sigma factor:

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1119 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1271 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Stephen W White, Jie Zheng, Yong-Mei Zhang, Rock
The structural biology of type II fatty acid biosynthesis.
Annu Rev Biochem: 2005, 74;791-831
[PubMed:15952903] [WorldCat.org] [DOI] (P p)

John E Cronan, Grover L Waldrop
Multi-subunit acetyl-CoA carboxylases.
Prog Lipid Res: 2002, 41(5);407-35
[PubMed:12121720] [WorldCat.org] [DOI] (P p)