Sandbox

• Description: glutamine-fructose-6-phosphate transaminase
  
  

Gene name glaube ich oder nicht	glmS
Synonyms	gcaA, ybxD
Essential	yes PubMed
Product	glutamine-fructose-6-phosphate transaminase
Function	cell wall synthesis
Metabolic function and regulation of this protein in SubtiPathways: sandbox
MW, pI	65 kDa, 4.796
Gene length, protein length	1800 bp, 600 aa
Immediate neighbours	glmM, ybbU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context File:Quintos.gif This image was kindly provided by SubtiList
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes

This gene is a member of the following regulons

glmS ribozyme

The gene

Basic information

• Locus tag: BSU01780

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: [HELLO BSU00100]
• BsubCyc: "

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: [HELLO BSU00100]
• BsubCyc: BSU00240

• Structure:
  • HIV2 (from Bacillus subtilis, 100% identity) PubMed
  • 2VF4 (GlmS from E. coli, 39% identity, 58% similarity) PubMed
  • the ribozyme: 3G8S, 3G9C, 3G8T, 3G95, 3G96 (all for the ribozyme from Bacillus anthracis), 2HO7 (the ribozyme from Thermonanaerobacter tengcongensis)

• UniProt: P39754

• KEGG entry: [2]

• E.C. number: 2.6.1.16

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: ybbP-ybbR-glmM-glmS

• Expression browser: glmS PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine PubMed
  • glmS is only expressed in the absence of glucosamine 6-phosphate (glmS ribozyme)

• Regulatory mechanism: glmS ribozyme: glucosamine 6-phosphate binds the leader mRNA, and a riboswitch with ribozyme activity cleaves off the glmS section from the mRNA, resulting in stopp of transcript elongation

• Additional information:
  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • A ncRNA is predicted between glmM and glmS PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2000 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 4000 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wade Winkler, University of Texas, USA, Homepage

References

Reviews

Philippe Durand, Béatrice Golinelli-Pimpaneau, Stéphane Mouilleron, Bernard Badet, Marie-Ange Badet-Denisot
Highlights of glucosamine-6P synthase catalysis.
Arch Biochem Biophys: 2008, 474(2);302-17
[PubMed:18279655] [WorldCat.org] [DOI] (I p)

Other Original Publications

Additional publications: PubMed