TagF

• Description: CDP-glycerol:polyglycerol phosphate glycero-phosphotransferase
  
  

Gene name	tagF
Synonyms	rodC
Essential	yes PubMed
Product	CDP-glycerol:polyglycerol phosphate glycero-phosphotransferase
Function	biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: tagF
Metabolic function and regulation of this protein in SubtiPathways: TagF
MW, pI	87 kDa, 9.4
Gene length, protein length	2238 bp, 746 aa
Immediate neighbours	tagG, tagE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes, membrane proteins

This gene is a member of the following regulons

PhoP regulon, WalR regulon

The gene

Basic information

• Locus tag: BSU35720

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU35720

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: CDP-glycerol + (glycerophosphate)(n) = CMP + (glycerophosphate)(n+1) (according to Swiss-Prot)

• Protein family: CDP-glycerol glycerophosphotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane associated PubMed

Database entries

• BsubCyc: BSU35720

• Structure:

• UniProt: P13485

• KEGG entry: [3]

• E.C. number: 2.7.8.12

Expression and regulation

• Operon: tagD-tagE-tagF PubMed

• Expression browser: tagF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by phosphate (PhoP) PubMed
  • strongly repressed in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed
  • WalR: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 168 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 693 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Imke G de Jong, Jan-Willem Veening, Oscar P Kuipers
Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation.
Environ Microbiol: 2012, 14(12);3110-21
[PubMed:23033921] [WorldCat.org] [DOI] (I p)

Edward W C Sewell, Mark P Pereira, Eric D Brown
The wall teichoic acid polymerase TagF is non-processive in vitro and amenable to study using steady state kinetic analysis.
J Biol Chem: 2009, 284(32);21132-8
[PubMed:19520862] [WorldCat.org] [DOI] (P p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Alex Formstone, Rut Carballido-López, Philippe Noirot, Jeffery Errington, Dirk-Jan Scheffers
Localization and interactions of teichoic acid synthetic enzymes in Bacillus subtilis.
J Bacteriol: 2008, 190(5);1812-21
[PubMed:18156271] [WorldCat.org] [DOI] (I p)

Jeffrey W Schertzer, Amit P Bhavsar, Eric D Brown
Two conserved histidine residues are critical to the function of the TagF-like family of enzymes.
J Biol Chem: 2005, 280(44);36683-90
[PubMed:16141206] [WorldCat.org] [DOI] (P p)

Amit P Bhavsar, Laura K Erdman, Jeffrey W Schertzer, Eric D Brown
Teichoic acid is an essential polymer in Bacillus subtilis that is functionally distinct from teichuronic acid.
J Bacteriol: 2004, 186(23);7865-73
[PubMed:15547257] [WorldCat.org] [DOI] (P p)

Alistair Howell, Sarah Dubrac, Kasper Krogh Andersen, David Noone, Juliette Fert, Tarek Msadek, Kevin Devine
Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach.
Mol Microbiol: 2003, 49(6);1639-55
[PubMed:12950927] [WorldCat.org] [DOI] (P p)

Jeffrey W Schertzer, Eric D Brown
Purified, recombinant TagF protein from Bacillus subtilis 168 catalyzes the polymerization of glycerol phosphate onto a membrane acceptor in vitro.
J Biol Chem: 2003, 278(20);18002-7
[PubMed:12637499] [WorldCat.org] [DOI] (P p)

W Liu, S Eder, F M Hulett
Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P.
J Bacteriol: 1998, 180(3);753-8
[PubMed:9457886] [WorldCat.org] [DOI] (P p)

C Mauël, A Bauduret, C Chervet, S Beggah, D Karamata
In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes.
Microbiology (Reading): 1995, 141 ( Pt 10);2379-89
[PubMed:7581998] [WorldCat.org] [DOI] (P p)

P M Wagner, G C Stewart
Role and expression of the Bacillus subtilis rodC operon.
J Bacteriol: 1991, 173(14);4341-6
[PubMed:1712357] [WorldCat.org] [DOI] (P p)

A L Honeyman, G C Stewart
The nucleotide sequence of the rodC operon of Bacillus subtilis.
Mol Microbiol: 1989, 3(9);1257-68
[PubMed:2507871] [WorldCat.org] [DOI] (P p)