TagE

• Description: UDP-glucose:polyglycerol phosphate glucosyltransferase
  
  

Gene name	tagE
Synonyms	rodD, gtaA, gtaD
Essential	no
Product	UDP-glucose:polyglycerol phosphate glucosyltransferase
Function	biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: tagE
Metabolic function and regulation of this protein in SubtiPathways: TagE
MW, pI	78 kDa, 6.026
Gene length, protein length	2019 bp, 673 aa
Immediate neighbours	tagF, tagD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, phosphoproteins

This gene is a member of the following regulons

PhoP regulon, WalR regulon

The gene

Basic information

• Locus tag: BSU35730

Phenotypes of a mutant

• inactivation of tagE is advantageous in environments with fluctuating glucose availability PubMed

Database entries

• BsubCyc: BSU35730

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transfer of glucose from UDP-glucose to glycerol phosphate in polymeric form PubMed

• Protein family: glycosyltransferase 1 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-2 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU35730

• Structure:

• UniProt: P13484

• KEGG entry: [3]

• E.C. number: 2.4.1.52

Expression and regulation

• Operon: tagD-tagE-tagF PubMed

• Expression browser: tagE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by phosphate (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed
  • WalR: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 64 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 346 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 411 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 573 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 491 PubMed

Biological materials

• Mutant:
  • 1A981 ( tagE::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Jeroen Siebring, Matthijs J H Elema, Fátima Drubi Vega, Akos T Kovács, Patsy Haccou, Oscar P Kuipers
Repeated triggering of sporulation in Bacillus subtilis selects against a protein that affects the timing of cell division.
ISME J: 2014, 8(1);77-87
[PubMed:23924781] [WorldCat.org] [DOI] (I p)

Sarah E Allison, Michael A D'Elia, Sharif Arar, Mario A Monteiro, Eric D Brown
Studies of the genetics, function, and kinetic mechanism of TagE, the wall teichoic acid glycosyltransferase in Bacillus subtilis 168.
J Biol Chem: 2011, 286(27);23708-16
[PubMed:21558268] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Alistair Howell, Sarah Dubrac, Kasper Krogh Andersen, David Noone, Juliette Fert, Tarek Msadek, Kevin Devine
Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach.
Mol Microbiol: 2003, 49(6);1639-55
[PubMed:12950927] [WorldCat.org] [DOI] (P p)

W Liu, S Eder, F M Hulett
Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P.
J Bacteriol: 1998, 180(3);753-8
[PubMed:9457886] [WorldCat.org] [DOI] (P p)

C Mauël, A Bauduret, C Chervet, S Beggah, D Karamata
In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes.
Microbiology (Reading): 1995, 141 ( Pt 10);2379-89
[PubMed:7581998] [WorldCat.org] [DOI] (P p)

A L Honeyman, G C Stewart
The nucleotide sequence of the rodC operon of Bacillus subtilis.
Mol Microbiol: 1989, 3(9);1257-68
[PubMed:2507871] [WorldCat.org] [DOI] (P p)