yvrHb

168

two-component response regulator (OmpR family)

Locus: BSU_33221

Molecular weight: 27.00 kDa

Isoelectric point: 5.235

Protein length: 237 aa

Gene length: 714 bp

Function: regulation cell surface maintenance

Product: two-component response regulator (OmpR family)

Essential: no

Synonyms: yvrHb, yvrH

Genomic Context

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List of homologs in different organisms, belongs to COG0745 (Galperin et al., 2021)

Gene

Coordinates: 3,408,353  3,409,066

Phenotypes of a mutant

• unusual autolysis and higher susceptibility to cell envelope antibiotics (bacitracin, aztreonam, cefepime, fosfomycin)  PubMed

The protein

Catalyzed reaction/ biological activity

• transcription activator  PubMed

Protein family

• OmpR family of two-component response regulators

Domains

• Response regulatory domain (aa 5-119) (according to UniProt)

Structure

• 2OQR (PDB) (from Mycobacterium tuberculosis, 37% identity) PubMed
• P94504 (AlphaFold)

Modification

• phosphorylation (Asp) by YvrG  PubMed

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yvrHb-yvrG-yvrE

  Description: PubMed

Biological materials

Mutant

• MGNA-B050 (yvrH::erm), available at the NBRP B. subtilis, Japan
• BKE33221 (yvrHb::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTTTATGTTCCTTTCTG,  downstream forward: _UP4_CCAAATCCTGAAGGCAAGCG
• BKK33221 (yvrHb::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTTTATGTTCCTTTCTG,  downstream forward: _UP4_CCAAATCCTGAAGGCAAGCG

References

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