TagA

• Description: UDP-N-acetyl-D-mannosamine transferase
  
  

Gene name	tagA
Synonyms
Essential	yes PubMed, no PubMed
Product	UDP-N-acetyl-D-mannosamine transferase
Function	biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: tagA
Metabolic function and regulation of this protein in SubtiPathways: TagA
MW, pI	29 kDa, 10.133
Gene length, protein length	768 bp, 256 aa
Immediate neighbours	tagD, tagB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components

This gene is a member of the following regulons

PhoP regulon, WalR regulon

The gene

Basic information

• Locus tag: BSU35750

Phenotypes of a mutant

• essential PubMed, reduced growth rate, loss of rod-like morphology PubMed
• inactivation of tagA strongly restores beta-lactam resistance in a sigM mutant PubMed

Database entries

• BsubCyc: BSU35750

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-N-acetyl-D-mannosamine + N-acetyl-D-glucosaminyldiphosphoundecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol (according to Swiss-Prot)

• Protein family: glycosyltransferase 26 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• BsubCyc: BSU35750

• Structure:

• UniProt: P27620

• KEGG entry: [3]

• E.C. number: 2.4.1.187

Expression and regulation

• Operon: tagA-tagB PubMed

• Expression browser: tagA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed when phosphate is available (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed
  • WalR: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 67 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: