melC

168

ABC transporter for melibiose and raffinose (permease)

Locus: BSU_30290

Molecular weight: 30.22 kDa

Isoelectric point: 9.85

Protein length: 276 aa

Gene length: 831 bp

Function: uptake of melibiose and raffinose

Product: sugar ABC transporter

Essential: no

Synonyms: melC, amyC

Genomic Context

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List of homologs in different organisms, belongs to COG0395 (Galperin et al., 2021)

Gene

Coordinates: 3,100,031  3,100,861

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)

Domains

• ABC transmembrane type-1 domain (aa 69-261) (according to UniProt)

Structure

• 4TQU (PDB) (from Sphingomonas sp., 26% identity) PubMed
• O34518 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: melE-melD-melC-melA

  Description: PubMed

  Regulation

  • repressed by glucose (CcpA) PubMed

• Genes: melR-melE-melD-melC-melA

  Description: PubMed

  Regulation

  • repressed by glucose (CcpA) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • MelR: repression, PubMed, in melR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A798 (amyC::erm), available at the NBRP B. subtilis, Japan
• BKE30290 (melC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTTTTTGTACGGGCGG,  downstream forward: _UP4_GGTTAACATTAAAGGGGGAA
• BKK30290 (melC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTTTTTGTACGGGCGG,  downstream forward: _UP4_GGTTAACATTAAAGGGGGAA

References

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