BglS

• Description: endo-beta-1,3-1,4 glucanase
  
  

Gene name	bglS
Synonyms	bgl, licS
Essential	no
Product	endo-beta-1,3-1,4 glucanase
Function	lichenan degradation
Gene expression levels in SubtiExpress: bglS
Metabolic function and regulation of this protein in SubtiPathways: bglS
MW, pI	27 kDa, 6.482
Gene length, protein length	726 bp, 242 aa
Immediate neighbours	citH, licT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, sporulation/ other

This gene is a member of the following regulons

CcpA regulon, LicT regulon

The gene

Basic information

• Locus tag: BSU39070

Database entries

• BsubCyc: BSU39070

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 16 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• BsubCyc: BSU39070

• Structure: 3O5S

• UniProt: P04957

• KEGG entry: [3]

• E.C. number: 3.2.1.73

Expression and regulation

• Operon:
  • licT-bglS PubMed
  • bglS PubMed

• Expression browser: bglS PubMed

• Sigma factor:
  • licT: SigA PubMed

• Regulation:
  • repressed by glucose (3.1-fold) (CcpA) PubMed
  • expressed in the stationary phase (temporal activation) PubMed
  • weak induction by ß-glucosides (LicT) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • LicT: binding to an RNA switch results in transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant: GP427 (licT-bglS, erm), BGW7 (cat), both available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Zichen Luo, Qiuqiang Gao, Xinliang Li, Jie Bao
Cloning of LicB from Clostridium thermocellum and its efficient secretive expression of thermostable β-1,3-1,4-glucanase.
Appl Biochem Biotechnol: 2014, 173(2);562-70
[PubMed:24659048] [WorldCat.org] [DOI] (I p)

Revathi Masilamani, Om Prakash Sharma, Suresh Kumar Muthuvel, Sakthivel Natarajan
Cloning, expression of b-1,3-1,4 glucanase from Bacillus subtilis SU40 and the effect of calcium ion on the stability of recombinant enzyme: in vitro and in silico analysis.
Bioinformation: 2013, 9(19);958-62
[PubMed:24391357] [WorldCat.org] [DOI] (P e)

Junio Cota, Leandro C Oliveira, André R L Damásio, Ana P Citadini, Zaira B Hoffmam, Thabata M Alvarez, Carla A Codima, Vitor B P Leite, Glaucia Pastore, Mario de Oliveira-Neto, Mario T Murakami, Roberto Ruller, Fabio M Squina
Assembling a xylanase-lichenase chimera through all-atom molecular dynamics simulations.
Biochim Biophys Acta: 2013, 1834(8);1492-500
[PubMed:23459129] [WorldCat.org] [DOI] (P p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

K Schnetz, J Stülke, S Gertz, S Krüger, M Krieg, M Hecker, B Rak
LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family.
J Bacteriol: 1996, 178(7);1971-9
[PubMed:8606172] [WorldCat.org] [DOI] (P p)

S Krüger, J Stülke, M Hecker
Catabolite repression of beta-glucanase synthesis in Bacillus subtilis.
J Gen Microbiol: 1993, 139(9);2047-54
[PubMed:8245831] [WorldCat.org] [DOI] (P p)

J Stülke, R Hanschke, M Hecker
Temporal activation of beta-glucanase synthesis in Bacillus subtilis is mediated by the GTP pool.
J Gen Microbiol: 1993, 139(9);2041-5
[PubMed:8245830] [WorldCat.org] [DOI] (P p)

N Murphy, D J McConnell, B A Cantwell
The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase.
Nucleic Acids Res: 1984, 12(13);5355-67
[PubMed:6087283] [WorldCat.org] [DOI] (P p)