ThrC

• Description: threonine synthase
  
  

Gene name	thrC
Synonyms	thrB
Essential	no
Product	threonine synthase
Function	biosynthesis of threonine
Gene expression levels in SubtiExpress: thrC
Metabolic function and regulation of this protein in SubtiPathways: thrC
MW, pI	37 kDa, 5.185
Gene length, protein length	1056 bp, 352 aa
Immediate neighbours	thrB, hom
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, most abundant proteins

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU32250

Database entries

• BsubCyc: BSU32250

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: O-phospho-L-homoserine + H₂O = L-threonine + phosphate (according to Swiss-Prot)

• Protein family: serine/threonine dehydratase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU32250

• Structure: 1UIN (from Thermus thermophilus, 51% identity, 69% similarity) PubMed

• UniProt: P04990

• KEGG entry: [2]

• E.C. number: 4.2.3.1

Expression and regulation

• Operon: hom-thrC-thrB PubMed

• Expression browser: thrC PubMed

• Sigma factor:

• Regulation:
  • repressed by casamino acids PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 5810 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3216 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 4616 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1614 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2833 PubMed

Biological materials

• Mutant:
  • 1A773 ( thrC::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: