TagT

• Description: phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid
  
  

Gene name	ywtF
Synonyms
Essential	no
Product	phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
Function	transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
Gene expression levels in SubtiExpress: tagT
Interactions involving this protein in SubtInteract: TagT
MW, pI	35 kDa, 9.554
Gene length, protein length	966 bp, 322 aa
Immediate neighbours	ywtG, ywtE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU35840

Phenotypes of a mutant

• a tagT mutant has no phenoype, the triple tagT tagU tagV mutant is unable to grow under normal conditions PubMed

Database entries

• BsubCyc: BSU35840

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LytR-Cps2a-Psr family PubMed

• Paralogous protein(s): TagU, TagV

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Mg(2+) PubMed

• Effectors of protein activity:

• Interactions:
  • MreB-TagT PubMed

• Localization:
  • cytoplasmic membrane (with extracytoplasmic catalaytic domain) PubMed
  • extracellular (signal peptide) PubMed

Database entries

• BsubCyc: BSU35840

• Structure:
  • 3MEJ
  • 4DE9 PubMed

• UniProt: Q7WY78

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ywtF PubMed

• Expression browser: tagT PubMed

• Sigma factor: SigM Pubmed

• Regulation:
  • expressed at high salt concentrations (SigM) Pubmed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 105 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 258 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: