yfkA

168

putative Fe-S oxidoreductase, affects the level of Efp modification

Locus: BSU_07955

Molecular weight: 43.00 kDa

Isoelectric point: 5.923

Protein length: 373 aa

Gene length: 1122 bp

Function: control of Efp modification

Product: putative Fe-S oxidoreductase

Essential: no

Synonyms: yfkA

Genomic Context

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List of homologs in different organisms, belongs to COG0535 (Galperin et al., 2021)

Gene

Coordinates: 868,007  869,128

Phenotypes of a mutant

• inactivation suppresses the swarming defect of a ymfI mutant PubMed

The protein

Protein family

• Radical SAM superfamily (according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• O34400 (AlphaFold)

Expression and Regulation

Operons

• Genes: yfkA-yfkC

  Description: PubMed

Biological materials

Mutant

• MGNA-C348 (yfkA::erm), available at the NBRP B. subtilis, Japan
• BKE07955 (yfkA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACTATCTAACTCCTTT,  downstream forward: _UP4_TAGATGCGGATGAAAGAAAC
• BKK07955 (yfkA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACTATCTAACTCCTTT,  downstream forward: _UP4_TAGATGCGGATGAAAGAAAC

References

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