YurJ

• Description: aminosugar ABC transporter (ATP-binding protein)
  
  

Gene name	yurJ
Synonyms
Essential	no
Product	aminosugar ABC transporter (ATP-binding protein)
Function	uptake of sugar amines
Gene expression levels in SubtiExpress: yurJ
Interactions involving this protein in SubtInteract: YurJ
Metabolic function and regulation of this protein in SubtiPathways: yurJ
MW, pI	41 kDa, 6.071
Gene length, protein length	1101 bp, 367 aa
Immediate neighbours	yurI, frlR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, utilization of specific carbon sources, utilization of nitrogen sources other than amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU32550

Database entries

• BsubCyc: BSU32550

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on Ser-3 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YurJ-(FrlM-FrlN)-FrlO PubMed

• Localization: membrane associated (via FrlM-FrlN) PubMed

Database entries

• BsubCyc: BSU32550

• Structure:

• UniProt: O32151

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yurJ PubMed

• Sigma factor:

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 52 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boumediene Soufi, Chanchal Kumar, Florian Gnad, Matthias Mann, Ivan Mijakovic, Boris Macek
Stable isotope labeling by amino acids in cell culture (SILAC) applied to quantitative proteomics of Bacillus subtilis.
J Proteome Res: 2010, 9(7);3638-46
[PubMed:20509597] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)