YitJ

• Description: methionine synthase
  
  

Gene name	yitJ
Synonyms
Essential	no
Product	methionine synthase
Function	methionine biosynthesis
Gene expression levels in SubtiExpress: yitJ
Metabolic function and regulation of this protein in SubtiPathways: yitJ
MW, pI	67 kDa, 5.336
Gene length, protein length	1836 bp, 612 aa
Immediate neighbours	yitI, yitK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU11010

Database entries

• BsubCyc: BSU11010

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane associated PubMed

Database entries

• BsubCyc: BSU11010

• Structure:

• UniProt: O06745

• KEGG entry: [2]

• E.C. number: 2.1.1.13

Expression and regulation

• Operon: yitJ-yitI-yitH PubMed

• Expression browser: yitJ PubMed

• Sigma factor:

• Regulation:
  • induced by methionine starvation (S-box) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 894 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: