CspR

• Description: similar to tRNA (Um34/Cm34) methyltransferase
  
  

Gene name	cspR
Synonyms	ygaR
Essential	yes PubMed
Product	tRNA (Um34/Cm34) methyltransferase homolog
Function	translation
Gene expression levels in SubtiExpress: cspR
MW, pI	18 kDa, 6.054
Gene length, protein length	480 bp, 160 aa
Immediate neighbours	yhbB, yhbD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

The gene

Basic information

• Locus tag: BSU08930

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU08930

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • catalyzes the methyl transfer from S-adenosyl-L-methionine to tRNA(Leu)(CAA) and tRNA(Leu)(UAA) isoacceptors PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU08930

• Structure: 4JAK 4JAK (from E. coli, 45% identity, 72% similarity) PubMed

• UniProt: O31590

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: cspR PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 124 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ru-Juan Liu, Mi Zhou, Zhi-Peng Fang, Meng Wang, Xiao-Long Zhou, En-Duo Wang
The tRNA recognition mechanism of the minimalist SPOUT methyltransferase, TrmL.
Nucleic Acids Res: 2013, 41(16);7828-42
[PubMed:23804755] [WorldCat.org] [DOI] (I p)