TagE

• Description: UDP-glucose:polyglycerol phosphate glucosyltransferase
  
  

Gene name	tagE
Synonyms	rodD, gtaA, gtaD
Essential	no
Product	UDP-glucose:polyglycerol phosphate glucosyltransferase
Function	biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: tagE
Metabolic function and regulation of this protein in SubtiPathways: TagE
MW, pI	78 kDa, 6.026
Gene length, protein length	2019 bp, 673 aa
Immediate neighbours	tagF, tagD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, phosphoproteins

This gene is a member of the following regulons

PhoP regulon, WalR regulon

The gene

Basic information

• Locus tag: BSU35730

Phenotypes of a mutant

• inactivation of tagE is advantageous in environments with fluctuating glucose availability PubMed

Database entries

• BsubCyc: BSU35730

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transfer of glucose from UDP-glucose to glycerol phosphate in polymeric form PubMed

• Protein family: glycosyltransferase 1 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-2 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU35730

• Structure:

• UniProt: P13484

• KEGG entry: [3]

• E.C. number: 2.4.1.52

Expression and regulation

• Operon: tagD-tagE-tagF PubMed

• Expression browser: tagE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by phosphate (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed
  • WalR: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 64 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 346 PubMed

Biological materials

• Mutant:
  • 1A981 ( tagE::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: