folC

168

folyl-polyglutamate synthetase

Locus: BSU_28080

Molecular weight: 48.00 kDa

Isoelectric point: 5.81

Protein length: 430 aa

Gene length: 1293 bp

Function: biosynthesis of folate

Product: folyl-polyglutamate synthetase

Essential: yes

E.C.: 6.3.2.17

Synonyms: folC

Genomic Context

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List of homologs in different organisms, belongs to COG0285 (Galperin et al., 2021)

Gene

Coordinates: 2,865,312  2,866,604

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• 7,8-dihydropteroate + ATP + L-glutamate --> 7,8-dihydrofolate + ADP + H⁺ + phosphate (according to UniProt)
• (6S)-5,6,7,8-tetrahydrofolyl-(γ-L-Glu)_n + ATP + L-glutamate --> (6S)-5,6,7,8-tetrahydrofolyl-(γ-L-Glu)_n+1 + ADP + H⁺ + phosphate (according to UniProt)

Protein family

• Folylpolyglutamate synthase family (single member, according to UniProt)

Structure

• 1FGS (PDB) (from Lactobacillus casei, 32% identity, 51% similarity) PubMed
• Q05865 (AlphaFold)

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: valS-folC

  Description: PubMed

  Regulation

  • induced by valine limitation (T-box) PubMed
  • the T-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • T-box: RNA switch, via riboswitch, in T-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

References

Reviews

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Original Publications

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