RpoA

• Description: RNA polymerase alpha subunit
  
  

Gene name	rpoA
Synonyms
Essential	yes PubMed
Product	RNA polymerase alpha subunit
Function	transcription
Gene expression levels in SubtiExpress: rpoA
Interactions involving this protein in SubtInteract: RpoA
MW, pI	34 kDa, 4.593
Gene length, protein length	942 bp, 314 aa
Immediate neighbours	rpsK, rplQ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, essential genes, most abundant proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU01430

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU01430

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1) (according to Swiss-Prot)

• Protein family: RNA polymerase alpha chain family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • RpoA-RpoB-RpoC PubMed
  • CcpN-RpoA PubMed
  • RpoA (C-terminal domain) -Spx PubMed
  • MgsR-RpoA PubMed
  • CcpA-RpoA PubMed
  • RpoA-CodY PubMed

• Localization:

Database entries

• BsubCyc: BSU01430

• Structure: 1Z3E (C-terminal domain, complex with Spx)

• UniProt: P20429

• KEGG entry: [2]

• E.C. number: 2.7.7.6

Expression and regulation

• Operon:

• Expression browser: rpoA PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 3504 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 11832 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

References

Reviews

Lakshminarayan M Iyer, L Aravind
Insights from the architecture of the bacterial transcription apparatus.
J Struct Biol: 2012, 179(3);299-319
[PubMed:22210308] [WorldCat.org] [DOI] (I p)