Rho

• Description: transcriptional termination protein
  
  

Gene name	rho
Synonyms
Essential	no
Product	transcriptional termination protein
Function	transcriptional termination
Gene expression levels in SubtiExpress: rho
Interactions involving this protein in SubtInteract: Rho
MW, pI	48 kDa, 6.865
Gene length, protein length	1281 bp, 427 aa
Immediate neighbours	rpmE, glpX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription

The gene

Basic information

• Locus tag: BSU37080

Database entries

• BsubCyc: BSU37080

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: rho family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Rho-NusG (C-terminal domain) (based on data for E. coli) PubMed

• Localization:

Database entries

• BsubCyc: BSU37080

• Structure: 1PVO (Rho from E. coli) PubMed

• UniProt: Q03222

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: rho PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 154 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 477 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

François D'Heygère, Makhlouf Rabhi, Marc Boudvillain
Phyletic distribution and conservation of the bacterial transcription termination factor Rho.
Microbiology (Reading): 2013, 159(Pt 7);1423-1436
[PubMed:23704790] [WorldCat.org] [DOI] (I p)

Marc Boudvillain, Nara Figueroa-Bossi, Lionello Bossi
Terminator still moving forward: expanding roles for Rho factor.
Curr Opin Microbiol: 2013, 16(2);118-24
[PubMed:23347833] [WorldCat.org] [DOI] (I p)

M Sofia Ciampi
Rho-dependent terminators and transcription termination.
Microbiology (Reading): 2006, 152(Pt 9);2515-2528
[PubMed:16946247] [WorldCat.org] [DOI] (P p)

John P Richardson
Loading Rho to terminate transcription.
Cell: 2003, 114(2);157-9
[PubMed:12887917] [WorldCat.org] [DOI] (P p)

J P Richardson
Structural organization of transcription termination factor Rho.
J Biol Chem: 1996, 271(3);1251-4
[PubMed:8576105] [WorldCat.org] [DOI] (P p)

Original publications

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Jisha Chalissery, Ghazala Muteeb, Nisha C Kalarickal, Shalini Mohan, V Jisha, Ranjan Sen
Interaction surface of the transcription terminator Rho required to form a complex with the C-terminal domain of the antiterminator NusG.
J Mol Biol: 2011, 405(1);49-64
[PubMed:21040729] [WorldCat.org] [DOI] (I p)

Vitaly Epshtein, Dipak Dutta, Joseph Wade, Evgeny Nudler
An allosteric mechanism of Rho-dependent transcription termination.
Nature: 2010, 463(7278);245-9
[PubMed:20075920] [WorldCat.org] [DOI] (I p)

Emmanuel Skordalakes, James M Berger
Structure of the Rho transcription terminator: mechanism of mRNA recognition and helicase loading.
Cell: 2003, 114(1);135-46
[PubMed:12859904] [WorldCat.org] [DOI] (P p)

H Yakhnin, J E Babiarz, A V Yakhnin, P Babitzke
Expression of the Bacillus subtilis trpEDCFBA operon is influenced by translational coupling and Rho termination factor.
J Bacteriol: 2001, 183(20);5918-26
[PubMed:11566991] [WorldCat.org] [DOI] (P p)

C J Ingham, J Dennis, P A Furneaux
Autogenous regulation of transcription termination factor Rho and the requirement for Nus factors in Bacillus subtilis.
Mol Microbiol: 1999, 31(2);651-63
[PubMed:10027981] [WorldCat.org] [DOI] (P p)